TY - JOUR AU - Funke, S.A. AU - Liu, H. AU - Sehl, T. AU - Bartnik, D. AU - Brener, O. AU - Nagel-Steger, L. AU - Wiesehan, K. AU - Willbold, D. TI - Identification and characterization of an Aß; oligomer precipitating peptide that may be useful to explore gene therapeutic approaches to Alzheimer's disease JO - Rejuvenation research VL - 15 SN - 1549-1684 CY - Larchmont, NY PB - Liebert M1 - PreJuSER-20882 SP - 144 - 147 PY - 2012 N1 - The authors gratefully acknowledge support from the "Helmholtz-CSC (China Scholarship Council) Junior Scientists Exchange Program" for Hongmei Liu. AB - A key feature of Alzheimer disease (AD) is the pathologic self-association of the amyloid-β (Aβ) peptide, leading to the formation of diffusible toxic Aβ oligomers and extracellular amyloid plaques. Next to extracellular Aβ, intraneuronal Aβ has important pathological functions in AD. Agents that specifically interfere with the oligomerization processes either outside or inside of neurons are highly desired for the elucidation of the pathologic mechanisms of AD and might even pave the way for new AD gene therapeutic approaches. Here, we characterize the Aβ binding peptide L3 and its influence on Aβ oligomerization in vitro. Preliminary studies in cell culture demonstrate that stably expressed L3 reduces cell toxicity of externally added Aβ in neuroblastoma cells. KW - Alzheimer Disease: genetics KW - Alzheimer Disease: therapy KW - Amyloid beta-Peptides: chemistry KW - Animals KW - Cell Line, Tumor KW - Centrifugation, Density Gradient KW - Disease Models, Animal KW - Gene Therapy: methods KW - Humans KW - Mice KW - Nephelometry and Turbidimetry KW - Neurons: metabolism KW - Peptides: chemistry KW - Sequence Analysis, DNA KW - Thiazoles: chemistry KW - Amyloid beta-Peptides (NLM Chemicals) KW - Peptides (NLM Chemicals) KW - Thiazoles (NLM Chemicals) KW - thioflavin T (NLM Chemicals) KW - J (WoSType) LB - PUB:(DE-HGF)16 C6 - pmid:22533419 UR - <Go to ISI:>//WOS:000303383600008 DO - DOI:10.1089/rej.2011.1262 UR - https://juser.fz-juelich.de/record/20882 ER -