TY  - JOUR
AU  - Granzin, J.
AU  - Huang, Y.
AU  - Topbas, C.
AU  - Huang, W.
AU  - Wu, Z.
AU  - Misra, S.
AU  - Hazen, S.L.
AU  - Blanton, R.E.
AU  - Lee, X.
AU  - Weiergräber, O. H.
TI  - Three-dimensional structure of a schistosome serpin revealing an unusual configuration of the helical subdomain
JO  - Acta crystallographica / D
VL  - 68
SN  - 0907-4449
CY  - Copenhagen
PB  - Munksgaard
M1  - PreJuSER-21147
SP  - 686 - 694
PY  - 2012
N1  - JG and OHW are grateful to Georg Buldt and Dieter Willbold for continuous generous support. XL and SH are supported by NIH grant P01HL076491. Furthermore, excellent assistance by Craig Ogata, the manager of the X4a beamline, and his staff at NSLS is greatly appreciated.
AB  - Parasitic organisms are constantly challenged by the defence mechanisms of their respective hosts, which often depend on serine protease activities. Consequently, protease inhibitors such as those belonging to the serpin superfamily have emerged as protective elements that support the survival of the parasites. This report describes the crystal structure of ShSPI, a serpin from the trematode Schistosoma haematobium. The protein is exposed on the surface of invading cercaria as well as of adult worms, suggesting its involvement in the parasite-host interaction. While generally conforming to the well established serpin fold, the structure reveals several distinctive features, mostly concerning the helical subdomain of the protein. It is proposed that these peculiarities are related to the unique biological properties of a small serpin subfamily which is conserved among pathogenic schistosomes.
KW  - Amino Acid Sequence
KW  - Animals
KW  - Humans
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Protein Structure, Secondary
KW  - Protein Structure, Tertiary
KW  - Schistosoma haematobium: chemistry
KW  - Sequence Alignment
KW  - Serpins: chemistry
KW  - Structural Homology, Protein
KW  - Serpins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22683791
C2  - pmc:PMC3370883
UR  - <Go to ISI:>//WOS:000305003700009
DO  - DOI:10.1107/S0907444912008372
UR  - https://juser.fz-juelich.de/record/21147
ER  -