TY  - JOUR
AU  - Möhl, C.
AU  - Kirchgeßner, N.
AU  - Schäfer, C.
AU  - Hoffmann, B.
AU  - Merkel, R.
TI  - Quantitative Mapping of Averaged Focal Adhesion Dynamics in Migrating Cells by Shape Normalization
JO  - Journal of cell science
VL  - 125
SN  - 0021-9533
CY  - Cambridge
PB  - Company of Biologists Limited
M1  - PreJuSER-21271
SP  - 155 - 165
PY  - 2012
N1  - This work was supported by the Bundesministerium fur Bildung und Forschung (BMBF) program [grant number 0315501].
AB  - The spatially ordered formation and disassembly of focal adhesions is a basic requirement for effective cell locomotion. Because focal adhesions couple the contractile actin-myosin network to the substrate, their distribution determines the pattern of traction forces propelling the cell in a certain direction. In the present study, we quantitatively analyzed the spatial patterning of cell-substrate adhesion in migrating cells by mapping averaged focal adhesion growth dynamics to a standardized cell coordinate system. These maps revealed distinct zones of focal adhesion assembly, disassembly and stability and were strongly interrelated with corresponding actin flow and traction force patterns. Moreover, the mapping technique enables precise detection of even minute responses of adhesion dynamics upon targeted signaling perturbations. For example, the partial inhibition of vinculin phosphorylation was followed by the reduced number of newly formed adhesions, whereas growth dynamics of existing adhesions remained unaffected.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22250204
UR  - <Go to ISI:>//WOS:000300329100015
DO  - DOI:10.1242/jcs.090746
UR  - https://juser.fz-juelich.de/record/21271
ER  -