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@ARTICLE{Monzel:21273,
      author       = {Monzel, C. and Fenz, SF. and Giesen, M. and Merkel, R. and
                      Sengupta, K.},
      title        = {{M}apping fluctuations in biomembranes adhered to
                      mictopatterns},
      journal      = {Soft matter},
      volume       = {8},
      issn         = {1744-683X},
      address      = {Cambridge},
      publisher    = {Royal Society of Chemistry (RSC)},
      reportid     = {PreJuSER-21273},
      pages        = {6128 - 6138},
      year         = {2012},
      note         = {We benefited highly from discussions with Ana-Suncana
                      Smith, Udo Seifert and Daniel Schmidt. We thank Dr Norbert
                      Kirchgessner and Sebastian Houben for helpful discussions on
                      Matlab and Nico Hampe for the fabrication of microstructured
                      SiO<INF>2</INF> wafers. One of the authors (C. M.) is
                      grateful for the financial support from the Bonn-Cologne
                      Graduate School and the Universite franco-allemande.},
      abstract     = {We studied biomembrane fluctuations by calculating the
                      instantaneous shape of model membranes adhered to
                      micro-patterned substrates, using micro-interferometry. The
                      model consisted of partially adherent giant unilamellar
                      vesicles (GUVs) which were osmotically deflated. Adhesion
                      was effected via the specific ligand-receptor interaction of
                      biotin-neutravidin. Special micro-structured adhesive
                      substrates were developed where the receptors were
                      distributed in the form of grids or lines. Dual-wavelength
                      reflection interference contrast microscopy (DW-RICM)
                      measurements revealed that on the structured adhesive
                      substrates GUVs exhibit regions of bound and fluctuating
                      membrane, in accordance with the underlying pattern. In the
                      fluctuating zone, the membrane presented itself as a
                      flat-topped hill for an initial osmotic difference of 70
                      mOsm l(-1). The membrane-substrate distance saturated at a
                      plateau of 79 +/- 9 nm. In this plateau, the fluctuation
                      amplitude was found to be 10 +/- 3 nm. Variation of the
                      shape (grid versus lines) or size (grids of 3.5 or 7 mu m
                      lattice constant) influenced neither the height nor the
                      fluctuation amplitude in the plateau. Fourier analysis
                      revealed that the mode corresponding to a wavelength of
                      twice the pattern size always contributed, and, depending on
                      the substrate, additional modes were sometimes present. The
                      plateau height could be tuned from 0 to 538 nm by changing
                      the initial osmotic gradient between the inside and outside
                      of the GUV, which effectively tuned the membrane tension.
                      The corresponding fluctuation amplitude ranged from
                      non-detectable to a maximum of 17 nm. Our results can be
                      interpreted in the light of a tension dependent effective
                      interaction potential.},
      keywords     = {J (WoSType)},
      cin          = {ICS-7},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung},
      pid          = {G:(DE-Juel1)FUEK505},
      shelfmark    = {Chemistry, Physical / Materials Science, Multidisciplinary
                      / Physics, Multidisciplinary / Polymer Science},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000304100900020},
      doi          = {10.1039/c2sm07458c},
      url          = {https://juser.fz-juelich.de/record/21273},
}