TY  - JOUR
AU  - Roscher, J.
AU  - Posch, T.N.
AU  - Pütz, M.
AU  - Huhn, C.
TI  - Forensic analysis of mesembrine alkaloids in Sceletium tortuosum by nonaqueous capillary electrophoresis mass spectrometry
JO  - Electrophoresis
VL  - 33
SN  - 0173-0835
CY  - Weinheim
PB  - Wiley-Blackwell
M1  - PreJuSER-21951
SP  - 1567 - 1570
PY  - 2012
N1  - We thank the Helmholtz Initiative and Networking Fund for financial support.
AB  - The consumption of legal and illegal drugs follows an organic trend comparable to the current trend in food consumption. The investigation of such drugs is therefore of interest to characterize the active ingredients of plants and drug preparations. A new method of nonaqueous capillary electrophoresis coupled to mass spectrometry (NACE-MS) as a powerful tool for the separation of complex alkaloid mixtures in difficult matrices is presented in this study for the analysis of samples of Sceletium tortuosum and drug products called Kanna made thereof. The method was found to be suitable for the investigation of the alkaloid composition and relative quantification of the ingredients. It proved of value to separate a large number of isobaric compounds, most probably including diastereomers, double-bond isomers, and further structurally closely related compounds. A comparison of plant samples from different vendors, self-fermented samples, and products ready for consumption was made. The high separation power obtained allowed a better description of the chemotypic differences of plant samples as well as Kanna preparations compared to other methods presented in the literature so far. Thus, the use of the NACE-MS enables a new perspective on the alkaloid profile of Sceletium species.
KW  - Electrophoresis, Capillary: methods
KW  - Fermentation
KW  - Forensic Sciences
KW  - Indole Alkaloids: analysis
KW  - Indole Alkaloids: chemistry
KW  - Indole Alkaloids: isolation & purification
KW  - Mass Spectrometry: methods
KW  - Mesembryanthemum: chemistry
KW  - Plant Extracts: analysis
KW  - Plant Extracts: chemistry
KW  - Plant Extracts: isolation & purification
KW  - Street Drugs: chemistry
KW  - Indole Alkaloids (NLM Chemicals)
KW  - Plant Extracts (NLM Chemicals)
KW  - Street Drugs (NLM Chemicals)
KW  - mesembrine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22736358
UR  - <Go to ISI:>//WOS:000305753600011
DO  - DOI:10.1002/elps.201100683
UR  - https://juser.fz-juelich.de/record/21951
ER  -