TY  - JOUR
AU  - Matusch, A.
AU  - Becker, J.S.
TI  - Bio-imaging of metals in a mouse model of Alzheimer's disease by laser ablation inductively coupled plasma mass spectrometry
JO  - Biomedical Spectroscopy and Imaging
VL  - 1
SN  - 2212-8794
CY  - Birmingham, Ala.
PB  - IOS Press
M1  - PreJuSER-22035
SP  - 57 - 65
PY  - 2012
N1  - Record converted from VDB: 12.11.2012
AB  - Quantitative mass spectrometry imaging (MSI) of metals in biological tissue sections by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using matrix-matched laboratory standards has been established previously. Here an improved calibration strategy is proposed, correcting for variable section thickness by normalization of sample and standard ion intensities to the average 13C+ ion signal of all ablated sample and standard material, respectively. Altered metal metabolism is known in Alzheimer's disease of which amyloid precursor protein (APP) transgenic mice are a popular model. Quantitative distribution analysis of Fe, Zn, Cu and Mn in horizontal brain sections of 45 week old Tg2576 transgenic mice showed a 22% decrease of Cu and changes of Fe, Zn and Mn of −1%, −7% and −17%. Local evaluation across a set of anatomically defined regions of interest showed an increased circum-ventricular to parenchyma ratio of Cu pointing to a higher Cu efflux and a heterogeneous pattern of Fe changes pointing to clearance of Fe at the sites of early pathology. Congruencies to synchrotron X-ray fluorescence data on distinct types of APP mice are discussed. MSI by LA-ICP-MS thus proved as a valuable and versatile tool for studying the cerebral metallo-architecture and the validation of animal models.
KW  - Adolescent
KW  - Adult
KW  - Bacteremia: epidemiology
KW  - Bacteremia: microbiology
KW  - Bacteria: isolation & purification
KW  - Child
KW  - Child, Preschool
KW  - Female
KW  - Gram-Negative Bacterial Infections: epidemiology
KW  - Gram-Negative Bacterial Infections: microbiology
KW  - Gram-Positive Bacterial Infections: epidemiology
KW  - Gram-Positive Bacterial Infections: microbiology
KW  - Humans
KW  - Incidence
KW  - Infant
KW  - Intestine, Small: transplantation
KW  - Male
KW  - Middle Aged
KW  - Postoperative Complications
KW  - Retrospective Studies
KW  - Risk Factors
KW  - Time Factors
KW  - Young Adult
LB  - PUB:(DE-HGF)16
C6  - pmid:22093913
DO  - DOI:10.3233/BSI-2012-0005
UR  - https://juser.fz-juelich.de/record/22035
ER  -