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000022169 0247_ $$2DOI$$a10.1074/jbc.M111.316729
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000022169 084__ $$2WoS$$aBiochemistry & Molecular Biology
000022169 1001_ $$0P:(DE-HGF)0$$aFerrante, P.$$b0
000022169 245__ $$aLHCBM1 and LHCBM2/7 polypeptides, components of the major LHCII complex, have distinct functional roles in the photosynthetic antenna system of Chlamydomonas reinhardtii
000022169 260__ $$aBethesda, Md.$$bSoc.$$c2012
000022169 300__ $$a16276 - 16288
000022169 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000022169 440_0 $$03091$$aJournal of Biological Chemistry$$v287$$x0021-9258$$y20
000022169 500__ $$aThis work was supported by the Italian Ministry of Agriculture, Hydrobio project, and by Euopean Union Project 245070 FP7-KBBE-2009-3 SUNBIOPATH.
000022169 520__ $$aThe photosystem II antenna of Chlamydomonas reinhardtii is composed of monomeric and trimeric complexes, the latter encoded by LHCBM genes. We employed artificial microRNA technology to specifically silence the LHCBM2 and LHCBM7 genes, encoding identical mature polypeptides, and the LHCBM1 gene. As a control, we studied the npq5 mutant, deficient in the LHCBM1 protein. The organization of LHCII complexes, functional antenna size, capacity for photoprotection, thermal energy dissipation and state transitions, and resistance to reactive oxygen species was studied in the various genotypes. Silencing of the LHCBM2/7 genes resulted in a decrease of an LHCII protein with an apparent molecular mass of 22 kDa, whereas silencing/lack of LHCBM1 caused the decrease/disappearance of a 23-kDa protein. A decrease in the abundance of trimeric LHCII complexes and in functional antenna size was observed in both LHCBM2/7 and LHCBM1 knockouts. In agreement with previous data, depletion of LHCBM1 decreased the capacity for excess energy dissipation but not the ability to perform state transitions. The opposite was true for LHCBM2/7, implying that this polypeptide has a different functional role from LHCBM1. The abundance of LHCBM1 and LHCBM2/7 is in both cases correlated with resistance to superoxide anion, whereas only LHCBM1 is also involved in singlet oxygen scavenging. These results suggest that different LHCBM components have well defined, non-redundant functions despite their high homology, implying that engineering of LHCBM proteins can be an effective strategy for manipulating the light harvesting system of Chlamydomonas reinhardtii.
000022169 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000022169 536__ $$0G:(EU-Grant)245070$$aSUNBIOPATH - Towards a better sunlight to biomass conversion efficiency in microalgae (245070)$$c245070$$fFP7-KBBE-2009-3$$x1
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000022169 650_2 $$2MeSH$$aChlamydomonas reinhardtii: enzymology
000022169 650_2 $$2MeSH$$aChlamydomonas reinhardtii: genetics
000022169 650_2 $$2MeSH$$aGene Silencing
000022169 650_2 $$2MeSH$$aMicroRNAs: genetics
000022169 650_2 $$2MeSH$$aMicroRNAs: metabolism
000022169 650_2 $$2MeSH$$aPhotosystem II Protein Complex: genetics
000022169 650_2 $$2MeSH$$aPhotosystem II Protein Complex: metabolism
000022169 650_2 $$2MeSH$$aPlant Proteins: genetics
000022169 650_2 $$2MeSH$$aPlant Proteins: metabolism
000022169 650_7 $$00$$2NLM Chemicals$$aMicroRNAs
000022169 650_7 $$00$$2NLM Chemicals$$aPhotosystem II Protein Complex
000022169 650_7 $$00$$2NLM Chemicals$$aPlant Proteins
000022169 650_7 $$2WoSType$$aJ
000022169 7001_ $$0P:(DE-HGF)0$$aBallottari, M.$$b1
000022169 7001_ $$0P:(DE-HGF)0$$aBonente, G.$$b2
000022169 7001_ $$0P:(DE-HGF)0$$aGiuliano, G.$$b3
000022169 7001_ $$0P:(DE-Juel1)VDB38860$$aBassi, R.$$b4$$uFZJ
000022169 773__ $$0PERI:(DE-600)1474604-9$$a10.1074/jbc.M111.316729$$gVol. 287, p. 16276 - 16288$$p16276 - 16288$$q287<16276 - 16288$$tThe @journal of biological chemistry$$v287$$x0021-9258$$y2012
000022169 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351333
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