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@ARTICLE{Ferrante:22169,
      author       = {Ferrante, P. and Ballottari, M. and Bonente, G. and
                      Giuliano, G. and Bassi, R.},
      title        = {{LHCBM}1 and {LHCBM}2/7 polypeptides, components of the
                      major {LHCII} complex, have distinct functional roles in the
                      photosynthetic antenna system of {C}hlamydomonas
                      reinhardtii},
      journal      = {The journal of biological chemistry},
      volume       = {287},
      issn         = {0021-9258},
      address      = {Bethesda, Md.},
      publisher    = {Soc.},
      reportid     = {PreJuSER-22169},
      pages        = {16276 - 16288},
      year         = {2012},
      note         = {This work was supported by the Italian Ministry of
                      Agriculture, Hydrobio project, and by Euopean Union Project
                      245070 FP7-KBBE-2009-3 SUNBIOPATH.},
      abstract     = {The photosystem II antenna of Chlamydomonas reinhardtii is
                      composed of monomeric and trimeric complexes, the latter
                      encoded by LHCBM genes. We employed artificial microRNA
                      technology to specifically silence the LHCBM2 and LHCBM7
                      genes, encoding identical mature polypeptides, and the
                      LHCBM1 gene. As a control, we studied the npq5 mutant,
                      deficient in the LHCBM1 protein. The organization of LHCII
                      complexes, functional antenna size, capacity for
                      photoprotection, thermal energy dissipation and state
                      transitions, and resistance to reactive oxygen species was
                      studied in the various genotypes. Silencing of the LHCBM2/7
                      genes resulted in a decrease of an LHCII protein with an
                      apparent molecular mass of 22 kDa, whereas silencing/lack of
                      LHCBM1 caused the decrease/disappearance of a 23-kDa
                      protein. A decrease in the abundance of trimeric LHCII
                      complexes and in functional antenna size was observed in
                      both LHCBM2/7 and LHCBM1 knockouts. In agreement with
                      previous data, depletion of LHCBM1 decreased the capacity
                      for excess energy dissipation but not the ability to perform
                      state transitions. The opposite was true for LHCBM2/7,
                      implying that this polypeptide has a different functional
                      role from LHCBM1. The abundance of LHCBM1 and LHCBM2/7 is in
                      both cases correlated with resistance to superoxide anion,
                      whereas only LHCBM1 is also involved in singlet oxygen
                      scavenging. These results suggest that different LHCBM
                      components have well defined, non-redundant functions
                      despite their high homology, implying that engineering of
                      LHCBM proteins can be an effective strategy for manipulating
                      the light harvesting system of Chlamydomonas reinhardtii.},
      keywords     = {Chlamydomonas reinhardtii: enzymology / Chlamydomonas
                      reinhardtii: genetics / Gene Silencing / MicroRNAs: genetics
                      / MicroRNAs: metabolism / Photosystem II Protein Complex:
                      genetics / Photosystem II Protein Complex: metabolism /
                      Plant Proteins: genetics / Plant Proteins: metabolism /
                      MicroRNAs (NLM Chemicals) / Photosystem II Protein Complex
                      (NLM Chemicals) / Plant Proteins (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {Terrestrische Umwelt / SUNBIOPATH - Towards a better
                      sunlight to biomass conversion efficiency in microalgae
                      (245070)},
      pid          = {G:(DE-Juel1)FUEK407 / G:(EU-Grant)245070},
      shelfmark    = {Biochemistry $\&$ Molecular Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22431727},
      pmc          = {pmc:PMC3351333},
      UT           = {WOS:000304030900020},
      doi          = {10.1074/jbc.M111.316729},
      url          = {https://juser.fz-juelich.de/record/22169},
}