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| 024 | 7 | _ | |2 pmid |a pmid:22431727 |
| 024 | 7 | _ | |2 pmc |a pmc:PMC3351333 |
| 024 | 7 | _ | |2 DOI |a 10.1074/jbc.M111.316729 |
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| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 100 | 1 | _ | |a Ferrante, P. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a LHCBM1 and LHCBM2/7 polypeptides, components of the major LHCII complex, have distinct functional roles in the photosynthetic antenna system of Chlamydomonas reinhardtii |
| 260 | _ | _ | |a Bethesda, Md. |b Soc. |c 2012 |
| 300 | _ | _ | |a 16276 - 16288 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Biological Chemistry |x 0021-9258 |0 3091 |y 20 |v 287 |
| 500 | _ | _ | |a This work was supported by the Italian Ministry of Agriculture, Hydrobio project, and by Euopean Union Project 245070 FP7-KBBE-2009-3 SUNBIOPATH. |
| 520 | _ | _ | |a The photosystem II antenna of Chlamydomonas reinhardtii is composed of monomeric and trimeric complexes, the latter encoded by LHCBM genes. We employed artificial microRNA technology to specifically silence the LHCBM2 and LHCBM7 genes, encoding identical mature polypeptides, and the LHCBM1 gene. As a control, we studied the npq5 mutant, deficient in the LHCBM1 protein. The organization of LHCII complexes, functional antenna size, capacity for photoprotection, thermal energy dissipation and state transitions, and resistance to reactive oxygen species was studied in the various genotypes. Silencing of the LHCBM2/7 genes resulted in a decrease of an LHCII protein with an apparent molecular mass of 22 kDa, whereas silencing/lack of LHCBM1 caused the decrease/disappearance of a 23-kDa protein. A decrease in the abundance of trimeric LHCII complexes and in functional antenna size was observed in both LHCBM2/7 and LHCBM1 knockouts. In agreement with previous data, depletion of LHCBM1 decreased the capacity for excess energy dissipation but not the ability to perform state transitions. The opposite was true for LHCBM2/7, implying that this polypeptide has a different functional role from LHCBM1. The abundance of LHCBM1 and LHCBM2/7 is in both cases correlated with resistance to superoxide anion, whereas only LHCBM1 is also involved in singlet oxygen scavenging. These results suggest that different LHCBM components have well defined, non-redundant functions despite their high homology, implying that engineering of LHCBM proteins can be an effective strategy for manipulating the light harvesting system of Chlamydomonas reinhardtii. |
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| 536 | _ | _ | |a SUNBIOPATH - Towards a better sunlight to biomass conversion efficiency in microalgae (245070) |0 G:(EU-Grant)245070 |c 245070 |x 1 |f FP7-KBBE-2009-3 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Chlamydomonas reinhardtii: enzymology |
| 650 | _ | 2 | |2 MeSH |a Chlamydomonas reinhardtii: genetics |
| 650 | _ | 2 | |2 MeSH |a Gene Silencing |
| 650 | _ | 2 | |2 MeSH |a MicroRNAs: genetics |
| 650 | _ | 2 | |2 MeSH |a MicroRNAs: metabolism |
| 650 | _ | 2 | |2 MeSH |a Photosystem II Protein Complex: genetics |
| 650 | _ | 2 | |2 MeSH |a Photosystem II Protein Complex: metabolism |
| 650 | _ | 2 | |2 MeSH |a Plant Proteins: genetics |
| 650 | _ | 2 | |2 MeSH |a Plant Proteins: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a MicroRNAs |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Photosystem II Protein Complex |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Plant Proteins |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Ballottari, M. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Bonente, G. |b 2 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Giuliano, G. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Bassi, R. |b 4 |u FZJ |0 P:(DE-Juel1)VDB38860 |
| 773 | _ | _ | |a 10.1074/jbc.M111.316729 |g Vol. 287, p. 16276 - 16288 |p 16276 - 16288 |q 287<16276 - 16288 |0 PERI:(DE-600)1474604-9 |t The @journal of biological chemistry |v 287 |y 2012 |x 0021-9258 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351333 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/22169/files/FZJ-22169.pdf |z Published final document. |y Restricted |
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