TY  - JOUR
AU  - Elmenhorst, D.
AU  - Aliaga, A.
AU  - Bauer, A.
AU  - Rosa-Neto, P.
TI  - Test-Retest Stability of Cerebral mGluR5 Quantification Using [11C]ABP688 and Positron Emission Tomography in Rats
JO  - Synapse
VL  - 66
SN  - 0887-4476
CY  - New York, NY
PB  - Wiley-Liss
M1  - PreJuSER-22269
SP  - 552 - 560
PY  - 2012
N1  - Contract grant sponsor: Heinrich Hertz Foundation of the Ministry of Science and Technology; Contract grant sponsor: North-Rhine Westfalia, Germany; Contract grant sponsor: Alzheimer's Association new investigator award; Contract grant number: NIRG-08-92090; Contract grant sponsor: Fonds de la Recherche en Sante du Quebec (FRSQ); Contract grant sponsor: Chercheur Burcier award (PRN); Contract grant sponsor: Aisenstadt Foundation.
AB  - This study evaluates the reproducibility of the quantification of metabotropic glutamate receptor type 5 (mGluR₅) densities in rats using the PET radiotracer [¹¹C]ABP688 and pharmacokinetic models that are based on an input function, which is derived from a reference tissue. Seven rats underwent dynamic PET scans (60 min) after bolus injection of [¹¹C]ABP688. Kinetic analyses included: binding potential (BP(ND) ) determined by calculating (a) the simplified reference tissue model (SRTM) and (b) its two-steps simplified version (SRTM2); (c) multilinear reference tissue model (MRTM) and (d) its 2-parameter version (MRTM2); (e) noninvasive graphical analysis (NIGA). Parametric images were generated representing BP(ND) by the MRTM2 model. BP(ND) determinations were reproducible with low to acceptable variability ranging from 5 to 10% and reproducibility scores (intraclass correlation coefficient) between 0.51 and 0.88. The pharmacokinetic model that showed lowest overall variability was the SRTM. In contrast, the use of the NIGA was associated with significantly lower reproducibility scores. Comparison of parametric images revealed no significant bias between test and retest measurements and is therefore suitable to compare groups at voxel levels. In conclusion, our results suggest that noninvasive quantification of [¹¹C]ABP688 imaging is reproducible and reliable for PET studies of the cerebral mGluR₅ in rats.
KW  - Algorithms
KW  - Animals
KW  - Brain: radionuclide imaging
KW  - Brain Chemistry
KW  - Carbon Radioisotopes: chemistry
KW  - Image Interpretation, Computer-Assisted
KW  - Kinetics
KW  - Male
KW  - Oximes: analysis
KW  - Oximes: chemistry
KW  - Positron-Emission Tomography: methods
KW  - Pyridines: analysis
KW  - Pyridines: chemistry
KW  - Rats
KW  - Rats, Sprague-Dawley
KW  - Receptors, Metabotropic Glutamate: analysis
KW  - Reproducibility of Results
KW  - 3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime (NLM Chemicals)
KW  - Carbon Radioisotopes (NLM Chemicals)
KW  - Oximes (NLM Chemicals)
KW  - Pyridines (NLM Chemicals)
KW  - Receptors, Metabotropic Glutamate (NLM Chemicals)
KW  - metabotropic glutamate receptor 5 (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22290765
UR  - <Go to ISI:>//WOS:000302293300009
DO  - DOI:10.1002/syn.21542
UR  - https://juser.fz-juelich.de/record/22269
ER  -