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@ARTICLE{Timm:22572,
author = {Timm, S. and Mielewczik, M. and Florian, A. and
Frankenbach, S. and Dreissen, A. and Hocken, N. and
Alisdair, R.F. and Fernie, A.R. and Walter, A. and Bauwe,
H.},
title = {{H}igh-to-{L}ow {CO}2 {A}cclimation {R}eveals {P}lasticity
of the {P}hotorespiratory {P}athway and {I}ndicates
{R}egulatory {L}inks to {C}ellular {M}etabolism of
{A}rabidopsis},
journal = {PLoS one},
volume = {7},
number = {8},
issn = {1932-6203},
address = {Lawrence, Kan.},
publisher = {PLoS},
reportid = {PreJuSER-22572},
pages = {e42809},
year = {2012},
note = {This work was supported by grants from the Deutsche
Forschungsgemeinschaft (Photorespiration Network PROMICS,
FOR 1186) to AW, ARF and HB). The funders had no role in
study design, data collection and analysis, decision to
publish, or preparation of the manuscript.},
abstract = {Photorespiratory carbon metabolism was long considered as
an essentially closed and nonregulated pathway with little
interaction to other metabolic routes except nitrogen
metabolism and respiration. Most mutants of this pathway
cannot survive in ambient air and require CO(2)-enriched air
for normal growth. Several studies indicate that this CO(2)
requirement is very different for individual mutants,
suggesting a higher plasticity and more interaction of
photorespiratory metabolism as generally thought. To
understand this better, we examined a variety of high- and
low-level parameters at $1\%$ CO(2) and their alteration
during acclimation of wild-type plants and selected
photorespiratory mutants to ambient air.The wild type and
four photorespiratory mutants of Arabidopsis thaliana
(Arabidopsis) were grown to a defined stadium at $1\%$ CO(2)
and then transferred to normal air $(0.038\%$ CO(2)). All
other conditions remained unchanged. This approach allowed
unbiased side-by-side monitoring of acclimation processes on
several levels. For all lines, diel (24 h) leaf growth,
photosynthetic gas exchange, and PSII fluorescence were
monitored. Metabolite profiling was performed for the wild
type and two mutants. During acclimation, considerable
variation between the individual genotypes was detected in
many of the examined parameters, which correlated with the
position of the impaired reaction in the photorespiratory
pathway.Photorespiratory carbon metabolism does not operate
as a fully closed pathway. Acclimation from high to low
CO(2) was typically steady and consistent for a number of
features over several days, but we also found unexpected
short-term events, such as an intermittent very massive rise
of glycine levels after transition of one particular mutant
to ambient air. We conclude that photorespiration is
possibly exposed to redox regulation beyond known
substrate-level effects. Additionally, our data support the
view that 2-phosphoglycolate could be a key regulator of
photosynthetic-photorespiratory metabolism as a whole.},
keywords = {J (WoSType)},
cin = {IBG-2},
ddc = {500},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22912743},
pmc = {pmc:PMC3422345},
UT = {WOS:000308063700024},
doi = {10.1371/journal.pone.0042809},
url = {https://juser.fz-juelich.de/record/22572},
}