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000022817 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000022817 084__ $$2WoS$$aPhysics, Applied
000022817 084__ $$2WoS$$aPhysics, Condensed Matter
000022817 1001_ $$0P:(DE-Juel1)128743$$aWerner, C. F.$$b0$$uFZJ
000022817 245__ $$aNutrient concentration-sensitive microorganism-based biosensor
000022817 260__ $$aWeinheim$$bWiley-VCH$$c2012
000022817 300__ $$a900 - 904
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000022817 440_0 $$04913$$aPhysica Status Solidi A$$v209$$x0031-8965$$y5
000022817 500__ $$3POF3_Assignment on 2016-02-29
000022817 500__ $$aThe authors thank the German Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) and the 'Fachagentur Nachwachsende Rohstoffe e. V.' (FNR) for financial support of this work (Bio-LAPS).
000022817 520__ $$aThis work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised. (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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000022817 65320 $$2Author$$abiosensor
000022817 65320 $$2Author$$aextracellular acidification
000022817 65320 $$2Author$$aglucose
000022817 65320 $$2Author$$alight-addressable potentiometric sensor
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000022817 7001_ $$0P:(DE-HGF)0$$aGröbel, S.$$b1
000022817 7001_ $$0P:(DE-HGF)0$$aKrumbe, C.$$b2
000022817 7001_ $$0P:(DE-HGF)0$$aWagner, T.$$b3
000022817 7001_ $$0P:(DE-HGF)0$$aSelmer, T.$$b4
000022817 7001_ $$0P:(DE-HGF)0$$aYoshinobu, T.$$b5
000022817 7001_ $$0P:(DE-HGF)0$$aBaumann, M. E. M.$$b6
000022817 7001_ $$0P:(DE-HGF)0$$aKeusgen, M.$$b7
000022817 7001_ $$0P:(DE-Juel1)128727$$aSchöning, M. J.$$b8$$uFZJ
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000022817 8567_ $$uhttp://dx.doi.org/10.1002/pssa.201100801
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