TY  - JOUR
AU  - Werner, C. F.
AU  - Gröbel, S.
AU  - Krumbe, C.
AU  - Wagner, T.
AU  - Selmer, T.
AU  - Yoshinobu, T.
AU  - Baumann, M. E. M.
AU  - Keusgen, M.
AU  - Schöning, M. J.
TI  - Nutrient concentration-sensitive microorganism-based biosensor
JO  - Physica status solidi / A
VL  - 209
IS  - 5
SN  - 0031-8965
CY  - Weinheim
PB  - Wiley-VCH
M1  - PreJuSER-22817
SP  - 900 - 904
PY  - 2012
N1  - The authors thank the German Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) and the 'Fachagentur Nachwachsende Rohstoffe e. V.' (FNR) for financial support of this work (Bio-LAPS).
AB  - This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised. (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000303386900017
DO  - DOI:10.1002/pssa.201100801
UR  - https://juser.fz-juelich.de/record/22817
ER  -