TY  - JOUR
AU  - Polen, T.
AU  - Rittmann, D.
AU  - Wendisch, V. F.
AU  - Sahm, H.
TI  - DNA microarray analyses of the long-term adaptive response of Escherichia coli to acetate and propionate
JO  - Applied and environmental microbiology
VL  - 69
SN  - 0099-2240
CY  - Washington, DC [u.a.]
PB  - Soc.
M1  - PreJuSER-22961
SP  - 1759 - 1774
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - In its natural environment, Escherichia coli is exposed to short-chain fatty acids, such as acetic acid or propionic acid, which can be utilized as carbon sources but which inhibit growth at higher concentrations. DNA microarray experiments revealed expression changes during exponential growth on complex medium due to the presence of sodium acetate or sodium propionate at a neutral external pH. The adaptive responses to acetate and propionate were similar and involved genes in three categories. First, the RNA levels for chemotaxis and flagellum genes increased. Accordingly, the expression of chromosomal fliC'-'lacZ and flhDC'-'lacZ fusions and swimming motility increased after adaptation to acetate or propionate. Second, the expression of many genes that are involved in the uptake and utilization of carbon sources decreased, indicating some kind of catabolite repression by acetate and propionate. Third, the expression of some genes of the general stress response increased, but the increases were more pronounced after short-term exposure for this response than for the adaptive response. Adaptation to propionate but not to acetate involved increased expression of threonine and isoleucine biosynthetic genes. The gene expression changes after adaptation to acetate or propionate were not caused solely by uncoupling or osmotic effects but represented specific characteristics of the long-term response of E. coli to either compound.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000181435600055
DO  - DOI:10.1128/AEM.69.3.1759-1774.2003
UR  - https://juser.fz-juelich.de/record/22961
ER  -