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@ARTICLE{Lange:22962,
      author       = {Lange, C. and Rittmann, D. and Wendisch, V. F. and Bott, M.
                      and Sahm, H.},
      title        = {{G}lobal expression profiling and physiological
                      characterization of {C}orynebacterium glutamicum grown in
                      the presence of {L}-valine},
      journal      = {Applied and environmental microbiology},
      volume       = {69},
      issn         = {0099-2240},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {PreJuSER-22962},
      pages        = {2521 - 2532},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Addition of L-valine (50 to 200 mM) to glucose minimal
                      medium had no effect on the growth of wild-type
                      Corynebacterium glutamicum ATCC 13032 but inhibited the
                      growth of the derived valine production strain VAL1 [13032
                      DeltailvA DeltapanBC(pJCilvBNCD)] in a
                      concentration-dependent manner. In order to explore this
                      strain-specific valine effect, genomewide expression
                      profiling was performed using DNA microarrays, which showed
                      that valine caused an increased ilvBN mRNA level in VAL1 but
                      not in the wild type. This unexpected result was confirmed
                      by an increased cellular level of the ilvB protein product,
                      i.e., the large subunit of acetohydroxyacid synthase (AHAS),
                      and by an increased AHAS activity of valine-treated VAL1
                      cells. The conclusion that valine caused the limitation of
                      another branched-chain amino acid was confirmed by showing
                      that high concentrations of L-isoleucine could relieve the
                      valine effect on VAL1 whereas L-leucine had the same effect
                      as valine. The valine-caused isoleucine limitation was
                      supported by the finding that the inhibitory valine effect
                      was linked to the ilvA deletion that results in isoleucine
                      auxotrophy. Taken together, these results implied that the
                      valine effect is caused by competition for uptake of
                      isoleucine by the carrier BrnQ, which transports all
                      branched-chained amino acids. Indeed, valine inhibition
                      could also be relieved by supplementing VAL1 with the
                      dipeptide isoleucyl-isoleucine, which is taken up by a
                      dipeptide transport system rather than by BrnQ.
                      Interestingly, addition of external valine stimulated valine
                      production by VAL1. This effect is most probably due to a
                      reduced carbon usage for biomass production and to the
                      increased expression of ilvBN, indicating that AHAS activity
                      may still be a limiting factor for valine production in the
                      VAL1 strain.},
      keywords     = {J (WoSType)},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK256},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000182808300012},
      pubmed       = {pmid:12732517},
      doi          = {10.1128/AEM.69.5.2521-2532.2003},
      url          = {https://juser.fz-juelich.de/record/22962},
}