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@ARTICLE{Lecher:23152,
author = {Lecher, J. and Schwarz, C.K.W. and Stoldt, M. and Smits,
S.H.J. and Willbold, D. and Schmitt, L.},
title = {{A}n {RTX} toxin transporters tether ist substrate prior to
secretion via the unique function of ist {N}- terminal
domain},
journal = {Structure},
volume = {20},
issn = {0969-2126},
address = {London [u.a.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-23152},
pages = {1778 - 1787},
year = {2012},
note = {Record converted from VDB: 12.11.2012},
abstract = {Type 1 secretion systems (T1SS) catalyze the one step
protein transport across the membranes of Gram-negative
bacteria and are composed of an outer membrane protein, a
membrane fusion protein and an ABC transporter. The ABC
transporter consists of the canonical nucleotide binding and
transmembrane domains. For the toxin hemolysin A (HlyA), the
ABC transporter HlyB carries an additional, N-terminal
domain sharing about $40\%$ homology to C39 peptidases, but
this "C39-like domain" (CLD) is suggested to feature
another, yet unknown function. Our functional and structural
analysis demonstrates that the CLD is essential for
secretion and that it specifically interacts with the
unfolded state of HlyA. We determined the nuclear magnetic
resonance structure of the CLD as well as the
substrate-binding region within the CLD. This mode of
action, represents a mechanism within T1SS and answers the
question, how a large and unfolded substrate is protected
inside the cells during secretion.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {Funktion und Dysfunktion des Nervensystems / BioSoft:
Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-Juel1)FUEK505},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22959622},
UT = {WOS:000309787900021},
doi = {10.1016/j.str.2012.08.005},
url = {https://juser.fz-juelich.de/record/23152},
}