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000023232 084__ $$2WoS$$aChemistry, Multidisciplinary
000023232 084__ $$2WoS$$aChemistry, Physical
000023232 084__ $$2WoS$$aNanoscience & Nanotechnology
000023232 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000023232 1001_ $$0P:(DE-HGF)0$$aKapralov, A.$$b0
000023232 245__ $$aSelective Adsorption of Surfactant Lipids by Single-Walled Carbon Nanotubes in Mouse Lung upon Pharyngeal Aspiration
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000023232 440_0 $$018823$$aACS Nano$$v6$$x1936-0851$$y5
000023232 500__ $$aThis work was supported by grants from National Institute for Occupational Safety and Health (NIOSH) OH008282, National Institutes of Health NIEHS R01ES019304, HL70755, HL094488, U19AI068021, ES021068-01, National Occupational Research Agenda NORA 0HELD015, 927000Y, 927Z1LU, Nanotechnology Research Center (NTRC) 927ZJHF, National Science Foundation (NSF) CAREER 0449117, seventh Framework Program of the European Commission (EC-FP7-NANOMMUNE-214281), and the Science Foundation of Ireland, Strategic Research Cluster (SRC) BioNanointeract and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Higher Education Authority (HEA) and Programme for Research in Third-Level Institutions (PRTLI), and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS), Cancer Center Support Grant (CCSG) P30 CA047904, and Environmental Protection Agency (EPA) FP-91713801. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health.
000023232 520__ $$aThe pulmonary route represents one of the most important portals of entry for nanoparticles into the body. However, the in vivo interactions of nanoparticles with biomolecules of the lung have not been sufficiently studied. Here, using an established mouse model of pharyngeal aspiration of single-walled carbon nanotubes (SWCNTs), we recovered SWCNTs from the bronchoalveolar lavage fluid (BALf), purified them from possible contamination with lung cells, and examined the composition of phospholipids adsorbed on SWCNTs by liquid chromatography mass spectrometry (LC-MS) analysis. We found that SWCNTs selectively adsorbed two types of the most abundant surfactant phospholipids: phosphatidylcholines (PC) and phosphatidylglycerols (PG). Molecular speciation of these phospholipids was also consistent with pulmonary surfactant. Quantitation of adsorbed lipids by LC-MS along with the structural assessments of phospholipid binding by atomic force microscopy and molecular modeling indicated that the phospholipids (∼108 molecules per SWCNT) formed an uninterrupted "coating" whereby the hydrophobic alkyl chains of the phospholipids were adsorbed onto the SWCNT with the polar head groups pointed away from the SWCNT into the aqueous phase. In addition, the presence of surfactant proteins A, B, and D on SWCNTs was determined by LC-MS. Finally, we demonstrated that the presence of this surfactant coating markedly enhanced the in vitro uptake of SWCNTs by macrophages. Taken together, this is the first demonstration of the in vivo adsorption of the surfactant lipids and proteins on SWCNTs in a physiologically relevant animal model.
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000023232 536__ $$0G:(EU-Grant)214281$$aNANOMMUNE - Comprehensive assessment of hazardous effects of engineered nanomaterials on the immune system (214281)$$c214281$$fFP7-NMP-2007-SMALL-1$$x1
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000023232 65320 $$2Author$$acarbon nanotubes
000023232 65320 $$2Author$$asurfactant
000023232 65320 $$2Author$$amacrophages
000023232 650_2 $$2MeSH$$aAdsorption
000023232 650_2 $$2MeSH$$aAnimals
000023232 650_2 $$2MeSH$$aLipids: chemistry
000023232 650_2 $$2MeSH$$aLung: metabolism
000023232 650_2 $$2MeSH$$aMice
000023232 650_2 $$2MeSH$$aNanotubes, Carbon
000023232 650_2 $$2MeSH$$aPharynx: metabolism
000023232 650_2 $$2MeSH$$aRespiratory Aspiration
000023232 650_2 $$2MeSH$$aSurface-Active Agents: chemistry
000023232 650_7 $$00$$2NLM Chemicals$$aLipids
000023232 650_7 $$00$$2NLM Chemicals$$aNanotubes, Carbon
000023232 650_7 $$00$$2NLM Chemicals$$aSurface-Active Agents
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000023232 7001_ $$0P:(DE-HGF)0$$aFeng, W.H.$$b1
000023232 7001_ $$0P:(DE-HGF)0$$aAmoscato, Andrew$$b2
000023232 7001_ $$0P:(DE-HGF)0$$aYanamala, N.$$b3
000023232 7001_ $$0P:(DE-HGF)0$$aBalasubramanian, K.$$b4
000023232 7001_ $$0P:(DE-HGF)0$$aWinnica, D.$$b5
000023232 7001_ $$0P:(DE-HGF)0$$aKisin, E.$$b6
000023232 7001_ $$0P:(DE-HGF)0$$aKotchey, G.$$b7
000023232 7001_ $$0P:(DE-HGF)0$$aGou, P.$$b8
000023232 7001_ $$0P:(DE-HGF)0$$aSparvero, L.$$b9
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000023232 7001_ $$0P:(DE-HGF)0$$aShvedova, A.$$b15
000023232 7001_ $$0P:(DE-HGF)0$$aKagan, V.$$b16
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