TY  - JOUR
AU  - Kapralov, A.
AU  - Feng, W.H.
AU  - Amoscato, Andrew
AU  - Yanamala, N.
AU  - Balasubramanian, K.
AU  - Winnica, D.
AU  - Kisin, E.
AU  - Kotchey, G.
AU  - Gou, P.
AU  - Sparvero, L.
AU  - Ray, P.
AU  - Mallampalli, R.
AU  - Klein-Seetharaman, J.
AU  - Fadeel, B.
AU  - Star, A.
AU  - Shvedova, A.
AU  - Kagan, V.
TI  - Selective Adsorption of Surfactant Lipids by Single-Walled Carbon Nanotubes in Mouse Lung upon Pharyngeal Aspiration
JO  - ACS nano
VL  - 6
SN  - 1936-0851
CY  - Washington, DC
PB  - Soc.
M1  - PreJuSER-23232
SP  - 4147 - 4156
PY  - 2012
N1  - This work was supported by grants from National Institute for Occupational Safety and Health (NIOSH) OH008282, National Institutes of Health NIEHS R01ES019304, HL70755, HL094488, U19AI068021, ES021068-01, National Occupational Research Agenda NORA 0HELD015, 927000Y, 927Z1LU, Nanotechnology Research Center (NTRC) 927ZJHF, National Science Foundation (NSF) CAREER 0449117, seventh Framework Program of the European Commission (EC-FP7-NANOMMUNE-214281), and the Science Foundation of Ireland, Strategic Research Cluster (SRC) BioNanointeract and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Higher Education Authority (HEA) and Programme for Research in Third-Level Institutions (PRTLI), and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS), Cancer Center Support Grant (CCSG) P30 CA047904, and Environmental Protection Agency (EPA) FP-91713801. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health.
AB  - The pulmonary route represents one of the most important portals of entry for nanoparticles into the body. However, the in vivo interactions of nanoparticles with biomolecules of the lung have not been sufficiently studied. Here, using an established mouse model of pharyngeal aspiration of single-walled carbon nanotubes (SWCNTs), we recovered SWCNTs from the bronchoalveolar lavage fluid (BALf), purified them from possible contamination with lung cells, and examined the composition of phospholipids adsorbed on SWCNTs by liquid chromatography mass spectrometry (LC-MS) analysis. We found that SWCNTs selectively adsorbed two types of the most abundant surfactant phospholipids: phosphatidylcholines (PC) and phosphatidylglycerols (PG). Molecular speciation of these phospholipids was also consistent with pulmonary surfactant. Quantitation of adsorbed lipids by LC-MS along with the structural assessments of phospholipid binding by atomic force microscopy and molecular modeling indicated that the phospholipids (∼108 molecules per SWCNT) formed an uninterrupted "coating" whereby the hydrophobic alkyl chains of the phospholipids were adsorbed onto the SWCNT with the polar head groups pointed away from the SWCNT into the aqueous phase. In addition, the presence of surfactant proteins A, B, and D on SWCNTs was determined by LC-MS. Finally, we demonstrated that the presence of this surfactant coating markedly enhanced the in vitro uptake of SWCNTs by macrophages. Taken together, this is the first demonstration of the in vivo adsorption of the surfactant lipids and proteins on SWCNTs in a physiologically relevant animal model.
KW  - Adsorption
KW  - Animals
KW  - Lipids: chemistry
KW  - Lung: metabolism
KW  - Mice
KW  - Nanotubes, Carbon
KW  - Pharynx: metabolism
KW  - Respiratory Aspiration
KW  - Surface-Active Agents: chemistry
KW  - Lipids (NLM Chemicals)
KW  - Nanotubes, Carbon (NLM Chemicals)
KW  - Surface-Active Agents (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22463369
C2  - pmc:PMC3358590
UR  - <Go to ISI:>//WOS:000304231700055
DO  - DOI:10.1021/nn300626q
UR  - https://juser.fz-juelich.de/record/23232
ER  -