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@ARTICLE{Kapralov:23232,
      author       = {Kapralov, A. and Feng, W.H. and Amoscato, Andrew and
                      Yanamala, N. and Balasubramanian, K. and Winnica, D. and
                      Kisin, E. and Kotchey, G. and Gou, P. and Sparvero, L. and
                      Ray, P. and Mallampalli, R. and Klein-Seetharaman, J. and
                      Fadeel, B. and Star, A. and Shvedova, A. and Kagan, V.},
      title        = {{S}elective {A}dsorption of {S}urfactant {L}ipids by
                      {S}ingle-{W}alled {C}arbon {N}anotubes in {M}ouse {L}ung
                      upon {P}haryngeal {A}spiration},
      journal      = {ACS nano},
      volume       = {6},
      issn         = {1936-0851},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {PreJuSER-23232},
      pages        = {4147 - 4156},
      year         = {2012},
      note         = {This work was supported by grants from National Institute
                      for Occupational Safety and Health (NIOSH) OH008282,
                      National Institutes of Health NIEHS R01ES019304, HL70755,
                      HL094488, U19AI068021, ES021068-01, National Occupational
                      Research Agenda NORA 0HELD015, 927000Y, 927Z1LU,
                      Nanotechnology Research Center (NTRC) 927ZJHF, National
                      Science Foundation (NSF) CAREER 0449117, seventh Framework
                      Program of the European Commission
                      (EC-FP7-NANOMMUNE-214281), and the Science Foundation of
                      Ireland, Strategic Research Cluster (SRC) BioNanointeract
                      and Centre for Research on Adaptive Nanostructures and
                      Nanodevices (CRANN), Higher Education Authority (HEA) and
                      Programme for Research in Third-Level Institutions (PRTLI),
                      and the Swedish Research Council for Environment,
                      Agricultural Sciences and Spatial Planning (FORMAS), Cancer
                      Center Support Grant (CCSG) P30 CA047904, and Environmental
                      Protection Agency (EPA) FP-91713801. The findings and
                      conclusions in this report are those of the authors and do
                      not necessarily represent the views of the National
                      Institute for Occupational Safety and Health.},
      abstract     = {The pulmonary route represents one of the most important
                      portals of entry for nanoparticles into the body. However,
                      the in vivo interactions of nanoparticles with biomolecules
                      of the lung have not been sufficiently studied. Here, using
                      an established mouse model of pharyngeal aspiration of
                      single-walled carbon nanotubes (SWCNTs), we recovered SWCNTs
                      from the bronchoalveolar lavage fluid (BALf), purified them
                      from possible contamination with lung cells, and examined
                      the composition of phospholipids adsorbed on SWCNTs by
                      liquid chromatography mass spectrometry (LC-MS) analysis. We
                      found that SWCNTs selectively adsorbed two types of the most
                      abundant surfactant phospholipids: phosphatidylcholines (PC)
                      and phosphatidylglycerols (PG). Molecular speciation of
                      these phospholipids was also consistent with pulmonary
                      surfactant. Quantitation of adsorbed lipids by LC-MS along
                      with the structural assessments of phospholipid binding by
                      atomic force microscopy and molecular modeling indicated
                      that the phospholipids (∼108 molecules per SWCNT) formed
                      an uninterrupted "coating" whereby the hydrophobic alkyl
                      chains of the phospholipids were adsorbed onto the SWCNT
                      with the polar head groups pointed away from the SWCNT into
                      the aqueous phase. In addition, the presence of surfactant
                      proteins A, B, and D on SWCNTs was determined by LC-MS.
                      Finally, we demonstrated that the presence of this
                      surfactant coating markedly enhanced the in vitro uptake of
                      SWCNTs by macrophages. Taken together, this is the first
                      demonstration of the in vivo adsorption of the surfactant
                      lipids and proteins on SWCNTs in a physiologically relevant
                      animal model.},
      keywords     = {Adsorption / Animals / Lipids: chemistry / Lung: metabolism
                      / Mice / Nanotubes, Carbon / Pharynx: metabolism /
                      Respiratory Aspiration / Surface-Active Agents: chemistry /
                      Lipids (NLM Chemicals) / Nanotubes, Carbon (NLM Chemicals) /
                      Surface-Active Agents (NLM Chemicals) / J (WoSType)},
      cin          = {ICS-5},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-5-20110106},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung / NANOMMUNE - Comprehensive
                      assessment of hazardous effects of engineered nanomaterials
                      on the immune system (214281)},
      pid          = {G:(DE-Juel1)FUEK505 / G:(EU-Grant)214281},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Nanoscience $\&$ Nanotechnology / Materials Science,
                      Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22463369},
      pmc          = {pmc:PMC3358590},
      UT           = {WOS:000304231700055},
      doi          = {10.1021/nn300626q},
      url          = {https://juser.fz-juelich.de/record/23232},
}