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000025196 0247_ $$2DOI$$a10.1103/PhysRevLett.89.028101
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000025196 084__ $$2WoS$$aPhysics, Multidisciplinary
000025196 1001_ $$0P:(DE-HGF)0$$aPrechtel, K.$$b0
000025196 245__ $$aDynamic force spectroscopy to probe adhesion strength of living cells
000025196 260__ $$aCollege Park, Md.$$bAPS$$c2002
000025196 300__ $$a028101-1 - 028101-4
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000025196 440_0 $$04925$$aPhysical Review Letters$$v89$$x0031-9007
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000025196 520__ $$aWe studied the mechanical strength of the adhesion of living cells to model membranes. The latter contained a RGD lipopeptide which is a high affinity binding site for a cell adhesion molecule (integrin alpha(V)beta(3)). Cells adhered specifically to the vesicles. We used micropipette aspiration for breaking this adhesion with well defined forces. Systematic variation of the rate of force application revealed pronounced kinetic effects. The dependence of the detachment forces on the loading rate was well described by a power law (exponent approximate to0.4), in agreement with recent theoretical work.
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000025196 7001_ $$0P:(DE-HGF)0$$aBausch, A. R.$$b1
000025196 7001_ $$0P:(DE-HGF)0$$aMarchi-Artzner, V.$$b2
000025196 7001_ $$0P:(DE-HGF)0$$aKantlehner, M.$$b3
000025196 7001_ $$0P:(DE-HGF)0$$aKessler, H.$$b4
000025196 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b5$$uFZJ
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