000255692 001__ 255692
000255692 005__ 20210129220524.0
000255692 0247_ $$2Handle$$a2128/16238
000255692 037__ $$aFZJ-2015-05824
000255692 041__ $$aEnglish
000255692 1001_ $$0P:(DE-Juel1)133468$$aDahmen, Volker$$b0$$ufzj
000255692 1112_ $$a15th International Congress of Radiation Reasearch$$cKyoto$$d2015-05-25 - 2015-05-29$$gICRR$$wJapan
000255692 245__ $$aInduction of the chromosomal translocation t(14;18) by targeting theBCL-2 locus with specific binding I-125-labeled Triplex-Forming oligonucleotides
000255692 260__ $$c2015
000255692 3367_ $$033$$2EndNote$$aConference Paper
000255692 3367_ $$2BibTeX$$aINPROCEEDINGS
000255692 3367_ $$2DRIVER$$aconferenceObject
000255692 3367_ $$2ORCID$$aCONFERENCE_POSTER
000255692 3367_ $$2DataCite$$aOutput Types/Conference Poster
000255692 3367_ $$0PUB:(DE-HGF)24$$2PUB:(DE-HGF)$$aPoster$$bposter$$mposter$$s1513261693_28640$$xAfter Call
000255692 520__ $$aTriplex-Forming oligonucleotides (TFO) are able to bind DNA in a sequence specific manner and are a promising tool to manipulate genes or gene regulatory units in a cellular environment. TFO possess a therapeutic potential e.g. as a carrier molecule for Alpha- or Auger-Electron-Emitter (AEE) to target specific DNA sequences in tumor cells. We established a method for the effective labeling of TFO with the AEE Iodine-125 (I-125) and analyzed the influence of I-125-labeled TFO in SCL-II cells on gene expression and translocation frequency of the human BCL2 gene. The TFO-BCL2 employed in the present study binds to the BCL2 gene at approximately 5400 bp upstream of the 3´-end. TFO labeling with I-125 was performed using the primer extension method. SCL-II cells were transfected with TFO via electroporation and subsequently stored at -150°C for decay accumulation up to a range from 100 to 330 decays/cell. SCL-II cells either transfected with I-125-labeled multi-binding TFO (>300,000 targets per genome) or transfected with non-labeled TFO-BCL2 served as controls. Monitoring of BCL2 translocations was done with the Fluorescence-In-Situ-Hybridization (FISH) method. The utilized FISH probes were designed to detect a translocation of the BCL2 gene from chromosome 18 to chromosome 14, a common translocation found in follicular lymphomas leading to an overexpression of BCL-2 protein. Analysis of BCL2 gene expression levels was done via quantitative Real-Time PCR on the Real Time PCR System 7500 (Applied Biosystems).The relative gene expression of BCl-2 in I-125-TFO-BCL2 transfected cells showed a significant up-regulation when compared to the controls. Control SCL-II cells were either transfected with I-125-labeled multi-binding TFO (>300,000 targets per genome) or non-labeled TFO-BCL2. Analysis of the BCL2 t(14;18) translocation frequency revealed a significant 1.8- to 2-fold increase when compared to the control cells.We conclude that I-125 decays within the BCL2 gene facilitates the occurrence of the t(14;18) chromosomal translocation in SCL-II cells and that the increased translocation frequency of t(14;18) contributes to the observed overall enhanced BCL-2 expression. However, it seems unlikely that these rare translocation events fully explain the observed enhanced gene expression of BCL-2.Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK005A
000255692 536__ $$0G:(DE-HGF)POF3-899$$a899 - ohne Topic (POF3-899)$$cPOF3-899$$fPOF III$$x0
000255692 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000255692 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b1$$eCorresponding author$$ufzj
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.pdf$$yOpenAccess
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.gif?subformat=icon$$xicon$$yOpenAccess
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000255692 8564_ $$uhttps://juser.fz-juelich.de/record/255692/files/Abstract_ICRR_Kyoto_2015.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000255692 909CO $$ooai:juser.fz-juelich.de:255692$$pdriver$$pVDB$$popen_access$$popenaire
000255692 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133468$$aForschungszentrum Jülich GmbH$$b0$$kFZJ
000255692 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133469$$aForschungszentrum Jülich GmbH$$b1$$kFZJ
000255692 9131_ $$0G:(DE-HGF)POF3-899$$1G:(DE-HGF)POF3-890$$2G:(DE-HGF)POF3-800$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000255692 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000255692 920__ $$lyes
000255692 9201_ $$0I:(DE-Juel1)S-US-20090406$$kS-US$$lSicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie$$x0
000255692 980__ $$aposter
000255692 980__ $$aVDB
000255692 980__ $$aUNRESTRICTED
000255692 980__ $$aI:(DE-Juel1)S-US-20090406
000255692 9801_ $$aFullTexts