TY  - JOUR
AU  - Ramalingam, Nagendran
AU  - Franke, Christof
AU  - Jaschinski, Evelin
AU  - Winterhoff, Moritz
AU  - Lu, Yao
AU  - Brühmann, Stefan
AU  - Junemann, Alexander
AU  - Meier, Helena
AU  - Noegel, Angelika A.
AU  - Weber, Igor
AU  - Zhao, Hongxia
AU  - Merkel, Rudolf
AU  - Schleicher, Michael
AU  - Faix, Jan
TI  - A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement
JO  - Nature Communications
VL  - 6
SN  - 2041-1723
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2015-05962
SP  - 8496 -
PY  - 2015
AB  - Cell migration is driven by the establishment of disparity between the cortical properties of the softer front and the more rigid rear allowing front extension and actomyosin-based rear contraction. However, how the cortical actin meshwork in the rear is generated remains elusive. Here we identify the mDia1-like formin A (ForA) from Dictyostelium discoideum that generates a subset of filaments as the basis of a resilient cortical actin sheath in the rear. Mechanical resistance of this actin compartment is accomplished by actin crosslinkers and IQGAP-related proteins, and is mandatory to withstand the increased contractile forces in response to mechanical stress by impeding unproductive blebbing in the rear, allowing efficient cell migration in two-dimensional-confined environments. Consistently, ForA supresses the formation of lateral protrusions, rapidly relocalizes to new prospective ends in repolarizing cells and is required for cortical integrity. Finally, we show that ForA utilizes the phosphoinositide gradients in polarized cells for subcellular targeting.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000363148700001
C6  - pmid:26415699
DO  - DOI:10.1038/ncomms9496
UR  - https://juser.fz-juelich.de/record/255856
ER  -