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@ARTICLE{Ramalingam:255856,
author = {Ramalingam, Nagendran and Franke, Christof and Jaschinski,
Evelin and Winterhoff, Moritz and Lu, Yao and Brühmann,
Stefan and Junemann, Alexander and Meier, Helena and Noegel,
Angelika A. and Weber, Igor and Zhao, Hongxia and Merkel,
Rudolf and Schleicher, Michael and Faix, Jan},
title = {{A} resilient formin-derived cortical actin meshwork in the
rear drives actomyosin-based motility in 2{D} confinement},
journal = {Nature Communications},
volume = {6},
issn = {2041-1723},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2015-05962},
pages = {8496 -},
year = {2015},
abstract = {Cell migration is driven by the establishment of disparity
between the cortical properties of the softer front and the
more rigid rear allowing front extension and
actomyosin-based rear contraction. However, how the cortical
actin meshwork in the rear is generated remains elusive.
Here we identify the mDia1-like formin A (ForA) from
Dictyostelium discoideum that generates a subset of
filaments as the basis of a resilient cortical actin sheath
in the rear. Mechanical resistance of this actin compartment
is accomplished by actin crosslinkers and IQGAP-related
proteins, and is mandatory to withstand the increased
contractile forces in response to mechanical stress by
impeding unproductive blebbing in the rear, allowing
efficient cell migration in two-dimensional-confined
environments. Consistently, ForA supresses the formation of
lateral protrusions, rapidly relocalizes to new prospective
ends in repolarizing cells and is required for cortical
integrity. Finally, we show that ForA utilizes the
phosphoinositide gradients in polarized cells for
subcellular targeting.},
cin = {ICS-7},
ddc = {500},
cid = {I:(DE-Juel1)ICS-7-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000363148700001},
pubmed = {pmid:26415699},
doi = {10.1038/ncomms9496},
url = {https://juser.fz-juelich.de/record/255856},
}