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| Hauptseite > Publikationsdatenbank > Single-Domain Peptidyl-Prolyl cis / trans Isomerase FkpA from Corynebacterium glutamicum Improves the Biomass Yield at Increased Growth Temperatures > print |
| Hauptseite > Publikationsdatenbank > Single-Domain Peptidyl-Prolyl cis / trans Isomerase FkpA from Corynebacterium glutamicum Improves the Biomass Yield at Increased Growth Temperatures > print |
| 001 | 256374 | ||
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| 100 | 1 | _ | |a Kallscheuer, Nicolai |0 P:(DE-Juel1)157678 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Single-Domain Peptidyl-Prolyl cis / trans Isomerase FkpA from Corynebacterium glutamicum Improves the Biomass Yield at Increased Growth Temperatures |
| 260 | _ | _ | |a Washington, DC [u.a.] |c 2015 |b Soc. |
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| 520 | _ | _ | |a Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the rate-limiting protein folding step at peptidyl bonds preceding proline residues and were found to be involved in several biological processes, including gene expression, signal transduction, and protein secretion. Representative enzymes were found in almost all sequenced genomes, including Corynebacterium glutamicum, a facultative anaerobic Gram-positive and industrial workhorse for the production of amino acids. In C. glutamicum, a predicted single-domain FK-506 (tacrolimus) binding protein (FKBP)-type PPIase (FkpA) is encoded directly downstream of gltA, which encodes citrate synthase (CS). This gene cluster is also present in other Actinobacteria. Here we carried out in vitro and in vivo experiments to study the function and influence of predicted FkpA in C. glutamicum. In vitro, FkpA indeed shows typical PPIase activity with artificial substrates and is inhibited by FK-506. Furthermore, FkpA delays the aggregation of CS, which is also inhibited by FK-506. Surprisingly, FkpA has a positive effect on the activity and temperature range of CS in vitro. Deletion of fkpA causes a 50% reduced biomass yield compared to that of the wild type when grown at 37°C, whereas there is only a 10% reduced biomass yield at the optimal growth temperature of 30°C accompanied by accumulation of 7 mM l-glutamate and 22 mM 2-oxoglutarate. Thus, FkpA may be exploited for improved product formation in biotechnical processes. Comparative transcriptome analysis revealed 69 genes which exhibit ≥2-fold mRNA level changes in C. glutamicum ΔfkpA, giving insight into the transcriptional response upon mild heat stress when FkpA is absent. |
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| 700 | 1 | _ | |a Polen, Tino |0 P:(DE-Juel1)128982 |b 3 |e Corresponding author |
| 773 | _ | _ | |a 10.1128/AEM.02113-15 |g Vol. 81, no. 22, p. 7839 - 7850 |0 PERI:(DE-600)1478346-0 |n 22 |p 7839 - 7850 |t Applied and environmental microbiology |v 81 |y 2015 |x 1098-5336 |
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