TY  - JOUR
AU  - Deptuła, Tobiasz
AU  - Buitenhuis, Johan
AU  - Jarzębski, Maciej
AU  - Patkowski, Adam
AU  - Gapinski, Jacek
TI  - Size of submicrometer particles measured by FCS: Correction of the confocal volume
JO  - Langmuir
VL  - 31
IS  - 24
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2015-06448
SP  - 6681 - 6687
PY  - 2015
AB  - When fluorescence correlation spectroscopy (FCS) in combination with a confocal microscope is used to determine the hydrodynamic radius a of particles comparable to or larger than the linear size σ of the confocal volume of the microscope, a correction must be used that depends on the a2/σ2 ratio and the distribution of the dye within the particle. Here we present the experimental validation of the theoretically predicted approximate correction necessary for appropriate measurements of the size of uniformly fluorescently labeled spheres of radius comparable to the size of the confocal volume. We also test the approximate correction formula for different ranges of the a/σ ratio and propose a simple procedure to obtain the correct nanoparticle size from such a measurement.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000356992000010
DO  - DOI:10.1021/acs.langmuir.5b01225
UR  - https://juser.fz-juelich.de/record/256564
ER  -