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@ARTICLE{Deptua:256564,
      author       = {Deptuła, Tobiasz and Buitenhuis, Johan and Jarzębski,
                      Maciej and Patkowski, Adam and Gapinski, Jacek},
      title        = {{S}ize of submicrometer particles measured by {FCS}:
                      {C}orrection of the confocal volume},
      journal      = {Langmuir},
      volume       = {31},
      number       = {24},
      issn         = {1520-5827},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2015-06448},
      pages        = {6681 - 6687},
      year         = {2015},
      abstract     = {When fluorescence correlation spectroscopy (FCS) in
                      combination with a confocal microscope is used to determine
                      the hydrodynamic radius a of particles comparable to or
                      larger than the linear size σ of the confocal volume of the
                      microscope, a correction must be used that depends on the
                      a2/σ2 ratio and the distribution of the dye within the
                      particle. Here we present the experimental validation of the
                      theoretically predicted approximate correction necessary for
                      appropriate measurements of the size of uniformly
                      fluorescently labeled spheres of radius comparable to the
                      size of the confocal volume. We also test the approximate
                      correction formula for different ranges of the a/σ ratio
                      and propose a simple procedure to obtain the correct
                      nanoparticle size from such a measurement.},
      cin          = {ICS-3},
      ddc          = {670},
      cid          = {I:(DE-Juel1)ICS-3-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000356992000010},
      doi          = {10.1021/acs.langmuir.5b01225},
      url          = {https://juser.fz-juelich.de/record/256564},
}