TY  - JOUR
AU  - Silin, F. H.
AU  - Wieder, H.
AU  - Woodward, J. T.
AU  - Valincius, G.
AU  - Offenhäusser, A.
AU  - Plant, A. L.
TI  - The role of surface free energy on the formation of hybrid bilayer membranes
JO  - Journal of the American Chemical Society
VL  - 124
SN  - 0002-7863
CY  - Washington, DC
PB  - American Chemical Society
M1  - PreJuSER-26705
SP  - 14676
PY  - 2002
N1  - Record converted from VDB: 12.11.2012
AB  - The interaction of small phospholipid vesicles with well-characterized surfaces has been studied to assess the effect of the surface free energy of the underlying monolayer on the formation of phospholipid/alkanethiol hybrid bilayer membranes (HBMs). The surface free energy was changed in a systematic manner using single-component alkanethiol monolayers and monolayers of binary mixtures of thiols. The binary surfaces were prepared on gold by self-assembly from binary solutions of the thiols HS-(CH(2))(n)()-X (n = 11, X = CH(3) or OH) in THF. Surface plasmon resonance (SPR), electrical capacitance, and atomic force microscopy (AFM) measurements were used to characterize the interaction of palmitoyl,oleoyl-phosphatidylcholine (POPC) vesicles with the surfaces. For all surfaces examined, it appears that the polar part of surface energy influences the nature of the POPC assembly that associates with the surface. Comparison of optical, capacitance, and AFM data suggests that vesicles can remain intact or partially intact even at surfaces with a contact angle with water of close to 100 degrees. In addition, comparison of the alkanethiols of different chain lengths and the fluorinated compound HS-(CH(2))(2)-(CF(2))(8)-CF(3) that characterize with a low value of the polar part of the surface energy suggests that the quality of the underlying monolayer in terms of number of defects has a significant influence on the packing density of the resulting HBM layer.
KW  - Adsorption
KW  - Alkanes: chemistry
KW  - Electric Capacitance
KW  - Hydrocarbons, Fluorinated: chemistry
KW  - Kinetics
KW  - Lipid Bilayers: chemistry
KW  - Membranes: chemistry
KW  - Microscopy, Atomic Force
KW  - Phosphatidylcholines: chemistry
KW  - Sulfhydryl Compounds: chemistry
KW  - Surface Plasmon Resonance
KW  - Surface Properties
KW  - Thermodynamics
KW  - Alkanes (NLM Chemicals)
KW  - Hydrocarbons, Fluorinated (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Phosphatidylcholines (NLM Chemicals)
KW  - Sulfhydryl Compounds (NLM Chemicals)
KW  - n-dodecane (NLM Chemicals)
KW  - 1-palmitoyl-2-oleoylphosphatidylcholine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12465979
UR  - <Go to ISI:>//WOS:000179661000040
DO  - DOI:10.1021/ja026585+
UR  - https://juser.fz-juelich.de/record/26705
ER  -