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@ARTICLE{Kottke:26841,
      author       = {Kottke, T. and Heberle, J. and Hehn, D. and Dick, B. and
                      Hegemann, P.},
      title        = {{P}hot-{LOV}1 : photocycle of a blue-light receptor domain
                      from the green alga {C}hlamydomonas reinhardtii},
      journal      = {Biophysical journal},
      volume       = {84},
      issn         = {0006-3495},
      address      = {New York, NY},
      publisher    = {Rockefeller Univ. Press},
      reportid     = {PreJuSER-26841},
      pages        = {1192 - 1201},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The "Phot" protein family comprises blue-light
                      photoreceptors that consist of two flavin mononucleotide
                      (FMN)binding LOV (light, oxygen, and voltage) domains and a
                      serine/threonine kinase domain. We have investigated the
                      LOV1 domain of Phot1 from Chlamydomonas reinhardtii by
                      time-resolved absorption spectroscopy. Photoexcitation of
                      the dark form, LOV1-447, causes transient bleaching and
                      formation of two spectrally similar red-shifted
                      intermediates that are both assigned to triplet states of
                      the FMN. The triplet states decay with time constants of 800
                      ns and 4 mus with an efficiency of $>90\%$ into a
                      blue-shifted intermediate, LOV1-390, that is attributed to a
                      thiol adduct of cysteine 57 to FMN C(4a). LOV1-390 reverts
                      to the dark form in hundreds of seconds, the time constant
                      being dependent on pH and salt concentration. In the mutant
                      C57S, where the thiol adduct cannot be formed, the triplet
                      state displays an oxygen-dependent decay directly to the
                      dark form. We present here a spectroscopic characterization
                      of an algal sensory photoreceptor in general and of a LOV1
                      domain photocycle in particular. The results are discussed
                      with respect to the behavior of the homologous LOV2 domain
                      from oat.},
      keywords     = {J (WoSType)},
      cin          = {IBI-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB58},
      pnm          = {Neurowissenschaften},
      pid          = {G:(DE-Juel1)FUEK255},
      shelfmark    = {Biophysics},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000183123700042},
      url          = {https://juser.fz-juelich.de/record/26841},
}