%0 Journal Article
%A Choe, B. J.
%A Jeong, D.-G.
%A Park, K. S.
%A Schlesinger, R.
%A Labahn, J.
%A Hofmann, K. P.
%A Büldt, G.
%T Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli : expression, crystallization and cryoconditions
%J Acta crystallographica / D
%V 59
%@ 0907-4449
%C Copenhagen
%I Munksgaard
%M PreJuSER-26893
%P 350 - 352
%D 2003
%Z Record converted from VDB: 12.11.2012
%X The gene for Escherichia coli ribonuclease P (RNase P) protein (also known as C5 protein) and its mutant C5-C113A have been expressed as GST fusion proteins in E. coli at a high level. After cleavage of the fusion protein, highly purified functional C5 protein is obtained that can be crystallized with 2.5-2.6 M (NH(4))(2)HPO(4)/(NH(4))H(2)PO(4) pH 7.0 at room temperature. These crystals are suitable for X-ray analysis, belong to the space group P3(1)21 or P3(2)21 (unit-cell parameters a = b = 66.67, c = 142.09 A) and diffract to 2.9 A at 100 K using sorbitol and glycerol as cryoprotectants. For three molecules in the asymmetric unit a V(M) of 2.17 A(3) Da(-1) was calculated.
%K Amino Acid Substitution
%K Cryoprotective Agents: chemistry
%K Crystallization
%K Crystallography, X-Ray
%K Endoribonucleases: chemistry
%K Endoribonucleases: genetics
%K Endoribonucleases: metabolism
%K Escherichia coli: enzymology
%K Escherichia coli Proteins
%K Glutathione Transferase: genetics
%K Glycerol: chemistry
%K RNA, Catalytic: chemistry
%K RNA, Catalytic: genetics
%K RNA, Catalytic: metabolism
%K Recombinant Fusion Proteins: biosynthesis
%K Recombinant Fusion Proteins: genetics
%K Ribonuclease P
%K Ribonucleoproteins: chemistry
%K Ribonucleoproteins: genetics
%K Ribonucleoproteins: metabolism
%K Sorbitol: chemistry
%K Cryoprotective Agents (NLM Chemicals)
%K Escherichia coli Proteins (NLM Chemicals)
%K RNA, Catalytic (NLM Chemicals)
%K Recombinant Fusion Proteins (NLM Chemicals)
%K Ribonucleoproteins (NLM Chemicals)
%K Sorbitol (NLM Chemicals)
%K Glycerol (NLM Chemicals)
%K Glutathione Transferase (NLM Chemicals)
%K Endoribonucleases (NLM Chemicals)
%K Ribonuclease P (NLM Chemicals)
%K ribonuclease P, E coli (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:12554950
%U <Go to ISI:>//WOS:000180641900020
%R 10.1107/S0907444902020784
%U https://juser.fz-juelich.de/record/26893