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000026893 0247_ $$2pmid$$apmid:12554950
000026893 0247_ $$2DOI$$a10.1107/S0907444902020784
000026893 0247_ $$2WOS$$aWOS:000180641900020
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000026893 041__ $$aeng
000026893 082__ $$a570
000026893 084__ $$2WoS$$aBiochemical Research Methods
000026893 084__ $$2WoS$$aBiochemistry & Molecular Biology
000026893 084__ $$2WoS$$aBiophysics
000026893 084__ $$2WoS$$aCrystallography
000026893 1001_ $$0P:(DE-HGF)0$$aChoe, B. J.$$b0
000026893 245__ $$aPreliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli : expression, crystallization and cryoconditions
000026893 260__ $$aCopenhagen$$bMunksgaard$$c2003
000026893 300__ $$a350 - 352
000026893 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000026893 3367_ $$00$$2EndNote$$aJournal Article
000026893 3367_ $$2BibTeX$$aARTICLE
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000026893 3367_ $$2DRIVER$$aarticle
000026893 440_0 $$056$$aActa Crystallographica D$$v59$$x0907-4449$$y2
000026893 500__ $$aRecord converted from VDB: 12.11.2012
000026893 520__ $$aThe gene for Escherichia coli ribonuclease P (RNase P) protein (also known as C5 protein) and its mutant C5-C113A have been expressed as GST fusion proteins in E. coli at a high level. After cleavage of the fusion protein, highly purified functional C5 protein is obtained that can be crystallized with 2.5-2.6 M (NH(4))(2)HPO(4)/(NH(4))H(2)PO(4) pH 7.0 at room temperature. These crystals are suitable for X-ray analysis, belong to the space group P3(1)21 or P3(2)21 (unit-cell parameters a = b = 66.67, c = 142.09 A) and diffract to 2.9 A at 100 K using sorbitol and glycerol as cryoprotectants. For three molecules in the asymmetric unit a V(M) of 2.17 A(3) Da(-1) was calculated.
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000026893 588__ $$aDataset connected to Web of Science, Pubmed
000026893 650_2 $$2MeSH$$aAmino Acid Substitution
000026893 650_2 $$2MeSH$$aCryoprotective Agents: chemistry
000026893 650_2 $$2MeSH$$aCrystallization
000026893 650_2 $$2MeSH$$aCrystallography, X-Ray
000026893 650_2 $$2MeSH$$aEndoribonucleases: chemistry
000026893 650_2 $$2MeSH$$aEndoribonucleases: genetics
000026893 650_2 $$2MeSH$$aEndoribonucleases: metabolism
000026893 650_2 $$2MeSH$$aEscherichia coli: enzymology
000026893 650_2 $$2MeSH$$aEscherichia coli Proteins
000026893 650_2 $$2MeSH$$aGlutathione Transferase: genetics
000026893 650_2 $$2MeSH$$aGlycerol: chemistry
000026893 650_2 $$2MeSH$$aRNA, Catalytic: chemistry
000026893 650_2 $$2MeSH$$aRNA, Catalytic: genetics
000026893 650_2 $$2MeSH$$aRNA, Catalytic: metabolism
000026893 650_2 $$2MeSH$$aRecombinant Fusion Proteins: biosynthesis
000026893 650_2 $$2MeSH$$aRecombinant Fusion Proteins: genetics
000026893 650_2 $$2MeSH$$aRibonuclease P
000026893 650_2 $$2MeSH$$aRibonucleoproteins: chemistry
000026893 650_2 $$2MeSH$$aRibonucleoproteins: genetics
000026893 650_2 $$2MeSH$$aRibonucleoproteins: metabolism
000026893 650_2 $$2MeSH$$aSorbitol: chemistry
000026893 650_7 $$00$$2NLM Chemicals$$aCryoprotective Agents
000026893 650_7 $$00$$2NLM Chemicals$$aEscherichia coli Proteins
000026893 650_7 $$00$$2NLM Chemicals$$aRNA, Catalytic
000026893 650_7 $$00$$2NLM Chemicals$$aRecombinant Fusion Proteins
000026893 650_7 $$00$$2NLM Chemicals$$aRibonucleoproteins
000026893 650_7 $$050-70-4$$2NLM Chemicals$$aSorbitol
000026893 650_7 $$056-81-5$$2NLM Chemicals$$aGlycerol
000026893 650_7 $$0EC 2.5.1.18$$2NLM Chemicals$$aGlutathione Transferase
000026893 650_7 $$0EC 3.1.-$$2NLM Chemicals$$aEndoribonucleases
000026893 650_7 $$0EC 3.1.26.5$$2NLM Chemicals$$aRibonuclease P
000026893 650_7 $$0EC 3.1.26.5$$2NLM Chemicals$$aribonuclease P, E coli
000026893 650_7 $$2WoSType$$aJ
000026893 7001_ $$0P:(DE-HGF)0$$aJeong, D.-G.$$b1
000026893 7001_ $$0P:(DE-HGF)0$$aPark, K. S.$$b2
000026893 7001_ $$0P:(DE-Juel1)VDB1421$$aSchlesinger, R.$$b3$$uFZJ
000026893 7001_ $$0P:(DE-Juel1)VDB886$$aLabahn, J.$$b4$$uFZJ
000026893 7001_ $$0P:(DE-HGF)0$$aHofmann, K. P.$$b5
000026893 7001_ $$0P:(DE-Juel1)131957$$aBüldt, G.$$b6$$uFZJ
000026893 773__ $$0PERI:(DE-600)2020492-9$$a10.1107/S0907444902020784$$gVol. 59, p. 350 - 352$$p350 - 352$$q59<350 - 352$$tActa crystallographica / D$$v59$$x0907-4449$$y2003
000026893 8567_ $$uhttp://dx.doi.org/10.1107/S0907444902020784
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000026893 9141_ $$y2003
000026893 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000026893 9201_ $$0I:(DE-Juel1)VDB58$$d31.12.2006$$gIBI$$kIBI-2$$lBiologische Strukturforschung$$x0
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