TY  - JOUR
AU  - Choe, B. J.
AU  - Jeong, D.-G.
AU  - Park, K. S.
AU  - Schlesinger, R.
AU  - Labahn, J.
AU  - Hofmann, K. P.
AU  - Büldt, G.
TI  - Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli : expression, crystallization and cryoconditions
JO  - Acta crystallographica / D
VL  - 59
SN  - 0907-4449
CY  - Copenhagen
PB  - Munksgaard
M1  - PreJuSER-26893
SP  - 350 - 352
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - The gene for Escherichia coli ribonuclease P (RNase P) protein (also known as C5 protein) and its mutant C5-C113A have been expressed as GST fusion proteins in E. coli at a high level. After cleavage of the fusion protein, highly purified functional C5 protein is obtained that can be crystallized with 2.5-2.6 M (NH(4))(2)HPO(4)/(NH(4))H(2)PO(4) pH 7.0 at room temperature. These crystals are suitable for X-ray analysis, belong to the space group P3(1)21 or P3(2)21 (unit-cell parameters a = b = 66.67, c = 142.09 A) and diffract to 2.9 A at 100 K using sorbitol and glycerol as cryoprotectants. For three molecules in the asymmetric unit a V(M) of 2.17 A(3) Da(-1) was calculated.
KW  - Amino Acid Substitution
KW  - Cryoprotective Agents: chemistry
KW  - Crystallization
KW  - Crystallography, X-Ray
KW  - Endoribonucleases: chemistry
KW  - Endoribonucleases: genetics
KW  - Endoribonucleases: metabolism
KW  - Escherichia coli: enzymology
KW  - Escherichia coli Proteins
KW  - Glutathione Transferase: genetics
KW  - Glycerol: chemistry
KW  - RNA, Catalytic: chemistry
KW  - RNA, Catalytic: genetics
KW  - RNA, Catalytic: metabolism
KW  - Recombinant Fusion Proteins: biosynthesis
KW  - Recombinant Fusion Proteins: genetics
KW  - Ribonuclease P
KW  - Ribonucleoproteins: chemistry
KW  - Ribonucleoproteins: genetics
KW  - Ribonucleoproteins: metabolism
KW  - Sorbitol: chemistry
KW  - Cryoprotective Agents (NLM Chemicals)
KW  - Escherichia coli Proteins (NLM Chemicals)
KW  - RNA, Catalytic (NLM Chemicals)
KW  - Recombinant Fusion Proteins (NLM Chemicals)
KW  - Ribonucleoproteins (NLM Chemicals)
KW  - Sorbitol (NLM Chemicals)
KW  - Glycerol (NLM Chemicals)
KW  - Glutathione Transferase (NLM Chemicals)
KW  - Endoribonucleases (NLM Chemicals)
KW  - Ribonuclease P (NLM Chemicals)
KW  - ribonuclease P, E coli (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12554950
UR  - <Go to ISI:>//WOS:000180641900020
DO  - DOI:10.1107/S0907444902020784
UR  - https://juser.fz-juelich.de/record/26893
ER  -