Home > Publications database > Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli : expression, crystallization and cryoconditions > print

001     26893
005     20200402210434.0
024 7 _ |2 pmid
|a pmid:12554950
024 7 _ |2 DOI
|a 10.1107/S0907444902020784
024 7 _ |2 WOS
|a WOS:000180641900020
037 _ _ |a PreJuSER-26893
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemical Research Methods
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
084 _ _ |2 WoS
|a Biophysics
084 _ _ |2 WoS
|a Crystallography
100 1 _ |a Choe, B. J.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli : expression, crystallization and cryoconditions
260 _ _ |a Copenhagen
|b Munksgaard
|c 2003
300 _ _ |a 350 - 352
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Acta Crystallographica D
|x 0907-4449
|0 56
|y 2
|v 59
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a The gene for Escherichia coli ribonuclease P (RNase P) protein (also known as C5 protein) and its mutant C5-C113A have been expressed as GST fusion proteins in E. coli at a high level. After cleavage of the fusion protein, highly purified functional C5 protein is obtained that can be crystallized with 2.5-2.6 M (NH(4))(2)HPO(4)/(NH(4))H(2)PO(4) pH 7.0 at room temperature. These crystals are suitable for X-ray analysis, belong to the space group P3(1)21 or P3(2)21 (unit-cell parameters a = b = 66.67, c = 142.09 A) and diffract to 2.9 A at 100 K using sorbitol and glycerol as cryoprotectants. For three molecules in the asymmetric unit a V(M) of 2.17 A(3) Da(-1) was calculated.
536 _ _ |a Neurowissenschaften
|c L01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK255
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Amino Acid Substitution
650 _ 2 |2 MeSH
|a Cryoprotective Agents: chemistry
650 _ 2 |2 MeSH
|a Crystallization
650 _ 2 |2 MeSH
|a Crystallography, X-Ray
650 _ 2 |2 MeSH
|a Endoribonucleases: chemistry
650 _ 2 |2 MeSH
|a Endoribonucleases: genetics
650 _ 2 |2 MeSH
|a Endoribonucleases: metabolism
650 _ 2 |2 MeSH
|a Escherichia coli: enzymology
650 _ 2 |2 MeSH
|a Escherichia coli Proteins
650 _ 2 |2 MeSH
|a Glutathione Transferase: genetics
650 _ 2 |2 MeSH
|a Glycerol: chemistry
650 _ 2 |2 MeSH
|a RNA, Catalytic: chemistry
650 _ 2 |2 MeSH
|a RNA, Catalytic: genetics
650 _ 2 |2 MeSH
|a RNA, Catalytic: metabolism
650 _ 2 |2 MeSH
|a Recombinant Fusion Proteins: biosynthesis
650 _ 2 |2 MeSH
|a Recombinant Fusion Proteins: genetics
650 _ 2 |2 MeSH
|a Ribonuclease P
650 _ 2 |2 MeSH
|a Ribonucleoproteins: chemistry
650 _ 2 |2 MeSH
|a Ribonucleoproteins: genetics
650 _ 2 |2 MeSH
|a Ribonucleoproteins: metabolism
650 _ 2 |2 MeSH
|a Sorbitol: chemistry
650 _ 7 |0 0
|2 NLM Chemicals
|a Cryoprotective Agents
650 _ 7 |0 0
|2 NLM Chemicals
|a Escherichia coli Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a RNA, Catalytic
650 _ 7 |0 0
|2 NLM Chemicals
|a Recombinant Fusion Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Ribonucleoproteins
650 _ 7 |0 50-70-4
|2 NLM Chemicals
|a Sorbitol
650 _ 7 |0 56-81-5
|2 NLM Chemicals
|a Glycerol
650 _ 7 |0 EC 2.5.1.18
|2 NLM Chemicals
|a Glutathione Transferase
650 _ 7 |0 EC 3.1.-
|2 NLM Chemicals
|a Endoribonucleases
650 _ 7 |0 EC 3.1.26.5
|2 NLM Chemicals
|a Ribonuclease P
650 _ 7 |0 EC 3.1.26.5
|2 NLM Chemicals
|a ribonuclease P, E coli
650 _ 7 |a J
|2 WoSType
700 1 _ |a Jeong, D.-G.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Park, K. S.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Schlesinger, R.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB1421
700 1 _ |a Labahn, J.
|b 4
|u FZJ
|0 P:(DE-Juel1)VDB886
700 1 _ |a Hofmann, K. P.
|b 5
|0 P:(DE-HGF)0
700 1 _ |a Büldt, G.
|b 6
|u FZJ
|0 P:(DE-Juel1)131957
773 _ _ |a 10.1107/S0907444902020784
|g Vol. 59, p. 350 - 352
|p 350 - 352
|q 59<350 - 352
|0 PERI:(DE-600)2020492-9
|t Acta crystallographica / D
|v 59
|y 2003
|x 0907-4449
856 7 _ |u http://dx.doi.org/10.1107/S0907444902020784
909 C O |o oai:juser.fz-juelich.de:26893
|p VDB
913 1 _ |k L01
|v Neurowissenschaften
|l Funktion und Dysfunktion des Nervensystems
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|x 0
914 1 _ |y 2003
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k IBI-2
|l Biologische Strukturforschung
|d 31.12.2006
|g IBI
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|x 0
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981 _ _ |a I:(DE-Juel1)ISB-2-20090406
981 _ _ |a I:(DE-Juel1)ICS-6-20110106

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