TY  - JOUR
AU  - Gardiennet, C.
AU  - Loquet, A.
AU  - Bockmann, A
AU  - Etzkorn, M.
AU  - Heise, H.
AU  - Baldus, M.
TI  - Structural constraints for the Crh protein from solid-state NMR experiments
JO  - Journal of biomolecular NMR
VL  - 40
SN  - 0925-2738
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - PreJuSER-275
SP  - 239 - 250
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - We demonstrate that short, medium and long-range constraints can be extracted from proton mediated, rare-spin detected correlation solid-state NMR experiments for the microcrystalline 10.4 x 2 kDa dimeric model protein Crh. Magnetization build-up curves from cross signals in NHHC and CHHC spectra deliver detailed information on side chain conformers and secondary structure for interactions between spin pairs. A large number of medium and long-range correlations can be observed in the spectra, and an analysis of the resolved signals reveals that the constraints cover the entire sequence, also including inter-monomer contacts between the two molecules forming the domain-swapped Crh dimer. Dynamic behavior is shown to have an impact on cross signals intensities, as indicated for mobile residues or regions by contacts predicted from the crystal structure, but absent in the spectra. Our work validates strategies involving proton distance measurements for large and complex proteins as the Crh dimer, and confirms the magnetization transfer properties previously described for small molecules in solid protein samples.
KW  - Bacterial Proteins: chemistry
KW  - Dimerization
KW  - Models, Molecular
KW  - Nuclear Magnetic Resonance, Biomolecular: methods
KW  - Phosphoproteins: chemistry
KW  - Protein Conformation
KW  - Protons
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Crh protein, Bacillus subtilis (NLM Chemicals)
KW  - Phosphoproteins (NLM Chemicals)
KW  - Protons (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18320329
C2  - pmc:PMC2579321
UR  - <Go to ISI:>//WOS:000254804800002
DO  - DOI:10.1007/s10858-008-9229-3
UR  - https://juser.fz-juelich.de/record/275
ER  -