TY  - JOUR
AU  - Heise, H.
AU  - Celej, M.S.
AU  - Becker, S.
AU  - Riedel, D.
AU  - Pelah, A.
AU  - Kumar, A.
AU  - Jovin, T.M.
AU  - Baldus, M.
TI  - Solid-state NMR reveals structural differences between fibrils of wild-type and disease-related A53T mutant alpha-synuclein
JO  - Journal of molecular biology
VL  - 380
SN  - 0022-2836
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PreJuSER-276
SP  - 444 - 450
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - Fibrils from the Parkinson's-disease-related A53T mutant of alpha-synuclein were investigated by solid-state NMR spectroscopy, electron microscopy, and atomic force microscopy. Sequential solid-state NMR resonance assignments were obtained for a large fraction of the fibril core. Experiments conducted above and below the freezing point suggest that the fibrils contain regions with increased mobility and structural elements different from beta-strand character, in addition to the rigid beta-sheet-rich core region. As in earlier studies on wild-type alpha-synuclein, the C-terminus was found to be flexible and unfolded, whereas the main core region was highly rigid and rich in beta-sheets. Compared to fibrils from wild-type alpha-synuclein, the well-ordered beta-sheet region extends to at least L38 and L100. These results demonstrate that a disease-related mutant of alpha-synuclein differs in both aggregation kinetics and fibril structure.
KW  - Amino Acid Sequence
KW  - Escherichia coli: genetics
KW  - Freezing
KW  - Humans
KW  - Microscopy, Atomic Force
KW  - Microscopy, Electron
KW  - Molecular Sequence Data
KW  - Mutation
KW  - Nuclear Magnetic Resonance, Biomolecular: methods
KW  - Parkinson Disease: genetics
KW  - Parkinson Disease: pathology
KW  - Protein Structure, Secondary
KW  - Recombinant Proteins: chemistry
KW  - Recombinant Proteins: metabolism
KW  - Recombinant Proteins: ultrastructure
KW  - alpha-Synuclein: chemistry
KW  - alpha-Synuclein: genetics
KW  - alpha-Synuclein: metabolism
KW  - alpha-Synuclein: ultrastructure
KW  - Recombinant Proteins (NLM Chemicals)
KW  - alpha-Synuclein (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18539297
UR  - <Go to ISI:>//WOS:000257564000002
DO  - DOI:10.1016/j.jmb.2008.05.026
UR  - https://juser.fz-juelich.de/record/276
ER  -