%0 Journal Article
%A Fisher, S. Zoë
%A von Schantz, Laura
%A Håkansson, Maria
%A Logan, Derek T.
%A Ohlin, Mats
%T Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand
%J Biochemistry
%V 54
%N 42
%@ 1520-4995
%C Columbus, Ohio
%I American Chemical Society
%M FZJ-2015-06688
%P 6435 - 6438
%D 2015
%X Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM–ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM–ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000363916200001
%$ pmid:26451738
%R 10.1021/acs.biochem.5b01058
%U https://juser.fz-juelich.de/record/276223