TY  - JOUR
AU  - Fisher, S. Zoë
AU  - von Schantz, Laura
AU  - Håkansson, Maria
AU  - Logan, Derek T.
AU  - Ohlin, Mats
TI  - Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand
JO  - Biochemistry
VL  - 54
IS  - 42
SN  - 1520-4995
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2015-06688
SP  - 6435 - 6438
PY  - 2015
AB  - Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM–ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM–ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000363916200001
C6  - pmid:26451738
DO  - DOI:10.1021/acs.biochem.5b01058
UR  - https://juser.fz-juelich.de/record/276223
ER  -