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@ARTICLE{Fisher:276223,
author = {Fisher, S. Zoë and von Schantz, Laura and Håkansson,
Maria and Logan, Derek T. and Ohlin, Mats},
title = {{N}eutron {C}rystallographic {S}tudies {R}eveal {H}ydrogen
{B}ond and {W}ater-{M}ediated {I}nteractions between a
{C}arbohydrate-{B}inding {M}odule and {I}ts {B}ound
{C}arbohydrate {L}igand},
journal = {Biochemistry},
volume = {54},
number = {42},
issn = {1520-4995},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {FZJ-2015-06688},
pages = {6435 - 6438},
year = {2015},
abstract = {Carbohydrate-binding modules (CBMs) are key components of
many carbohydrate-modifying enzymes. CBMs affect the
activity of these enzymes by modulating bonding and
catalysis. To further characterize and study CBM–ligand
binding interactions, neutron crystallographic studies of an
engineered family 4-type CBM in complex with a branched
xyloglucan ligand were conducted. The first neutron crystal
structure of a CBM–ligand complex reported here shows
numerous atomic details of hydrogen bonding and
water-mediated interactions and reveals the charged state of
key binding cleft amino acid side chains.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)BIODIFF-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000363916200001},
pubmed = {pmid:26451738},
doi = {10.1021/acs.biochem.5b01058},
url = {https://juser.fz-juelich.de/record/276223},
}