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100 1 _ |a Fisher, S. Zoë
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245 _ _ |a Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand
260 _ _ |a Columbus, Ohio
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520 _ _ |a Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM–ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM–ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.
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693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
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700 1 _ |a von Schantz, Laura
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700 1 _ |a Håkansson, Maria
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700 1 _ |a Logan, Derek T.
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700 1 _ |a Ohlin, Mats
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773 _ _ |a 10.1021/acs.biochem.5b01058
|g Vol. 54, no. 42, p. 6435 - 6438
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