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@ARTICLE{Michalczyk:276225,
author = {Michalczyk, Ryszard and Unkefer, Clifford J. and Bacik,
John-Paul and Schrader, Tobias E. and Ostermann, Andreas and
Kovalevsky, Andrey Y. and McKenna, Robert and Fisher,
Suzanne Zoë},
title = {{J}oint neutron crystallographic and {NMR} solution studies
of {T}yr residue ionization and hydrogen bonding:
{I}mplications for enzyme-mediated proton transfer},
journal = {Proceedings of the National Academy of Sciences of the
United States of America},
volume = {112},
number = {18},
issn = {1091-6490},
address = {Washington, DC},
publisher = {National Acad. of Sciences},
reportid = {FZJ-2015-06690},
pages = {5673 - 5678},
year = {2015},
abstract = {Proton transfer is a fundamental mechanism at the core of
many enzyme-catalyzed reactions. It is also exquisitely
sensitive to a number of factors, including pH,
electrostatics, proper active-site geometry, and chemistry.
Carbonic anhydrase has evolved a fast and efficient way to
conduct protons through a combination of hydrophilic amino
acid side chains that coordinate a highly ordered H-bonded
water network. This study uses a powerful approach,
combining NMR solution studies with neutron protein
crystallography, to determine the effect of pH and divalent
cations on key residues involved in proton transfer in human
carbonic anhydrase. The results have broad implications for
our understanding of proton transfer and how subtle changes
in ionization and H-bonding interactions can modulate enzyme
catalysis.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {000},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)BIODIFF-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000353953800049},
doi = {10.1073/pnas.1502255112},
url = {https://juser.fz-juelich.de/record/276225},
}