guest ::
| Home > Publications database > Joint neutron crystallographic and NMR solution studies of Tyr residue ionization and hydrogen bonding: Implications for enzyme-mediated proton transfer > print |
| Home > Publications database > Joint neutron crystallographic and NMR solution studies of Tyr residue ionization and hydrogen bonding: Implications for enzyme-mediated proton transfer > print |
| 001 | 276225 | ||
| 005 | 20240619091150.0 | ||
| 024 | 7 | _ | |a 10.1073/pnas.1502255112 |2 doi |
| 024 | 7 | _ | |a 0027-8424 |2 ISSN |
| 024 | 7 | _ | |a 1091-6490 |2 ISSN |
| 024 | 7 | _ | |a WOS:000353953800049 |2 WOS |
| 037 | _ | _ | |a FZJ-2015-06690 |
| 082 | _ | _ | |a 000 |
| 100 | 1 | _ | |a Michalczyk, Ryszard |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Joint neutron crystallographic and NMR solution studies of Tyr residue ionization and hydrogen bonding: Implications for enzyme-mediated proton transfer |
| 260 | _ | _ | |a Washington, DC |c 2015 |b National Acad. of Sciences |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1449472210_18272 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 520 | _ | _ | |a Proton transfer is a fundamental mechanism at the core of many enzyme-catalyzed reactions. It is also exquisitely sensitive to a number of factors, including pH, electrostatics, proper active-site geometry, and chemistry. Carbonic anhydrase has evolved a fast and efficient way to conduct protons through a combination of hydrophilic amino acid side chains that coordinate a highly ordered H-bonded water network. This study uses a powerful approach, combining NMR solution studies with neutron protein crystallography, to determine the effect of pH and divalent cations on key residues involved in proton transfer in human carbonic anhydrase. The results have broad implications for our understanding of proton transfer and how subtle changes in ionization and H-bonding interactions can modulate enzyme catalysis. |
| 536 | _ | _ | |0 G:(DE-HGF)POF3-6G15 |f POF III |x 0 |c POF3-6G15 |a 6G15 - FRM II / MLZ (POF3-6G15) |
| 536 | _ | _ | |a 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) |0 G:(DE-HGF)POF3-6G4 |c POF3-623 |f POF III |x 1 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
| 650 | 2 | 7 | |a Crystallography |0 V:(DE-MLZ)SciArea-240 |2 V:(DE-HGF) |x 1 |
| 650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-2016 |2 V:(DE-HGF) |x 1 |
| 650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-1 |2 V:(DE-HGF) |x 0 |
| 693 | _ | _ | |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz |e BIODIFF: Diffractometer for large unit cells |f NL1 |1 EXP:(DE-MLZ)FRMII-20140101 |0 EXP:(DE-MLZ)BIODIFF-20140101 |5 EXP:(DE-MLZ)BIODIFF-20140101 |6 EXP:(DE-MLZ)NL1-20140101 |x 0 |
| 700 | 1 | _ | |a Unkefer, Clifford J. |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Bacik, John-Paul |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Schrader, Tobias E. |0 P:(DE-Juel1)138266 |b 3 |u fzj |
| 700 | 1 | _ | |a Ostermann, Andreas |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Kovalevsky, Andrey Y. |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a McKenna, Robert |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Fisher, Suzanne Zoë |0 P:(DE-HGF)0 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1073/pnas.1502255112 |g Vol. 112, no. 18, p. 5673 - 5678 |0 PERI:(DE-600)1461794-8 |n 18 |p 5673 - 5678 |t Proceedings of the National Academy of Sciences of the United States of America |v 112 |y 2015 |x 1091-6490 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.pdf |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.gif?subformat=icon |x icon |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.jpg?subformat=icon-1440 |x icon-1440 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.jpg?subformat=icon-180 |x icon-180 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.jpg?subformat=icon-640 |x icon-640 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/276225/files/PNAS-2015-Michalczyk-5673-8-2.pdf?subformat=pdfa |x pdfa |y Restricted |
| 909 | C | O | |o oai:juser.fz-juelich.de:276225 |p VDB:MLZ |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)138266 |
| 913 | 1 | _ | |a DE-HGF |9 G:(DE-HGF)POF3-6G15 |x 0 |4 G:(DE-HGF)POF |v FRM II / MLZ |1 G:(DE-HGF)POF3-6G0 |0 G:(DE-HGF)POF3-6G15 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-600 |b Forschungsbereich Materie |l Großgeräte: Materie |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Von Materie zu Materialien und Leben |1 G:(DE-HGF)POF3-620 |0 G:(DE-HGF)POF3-623 |2 G:(DE-HGF)POF3-600 |v Facility topic: Neutrons for Research on Condensed Matter |9 G:(DE-HGF)POF3-6G4 |x 1 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2015 |
| 915 | _ | _ | |a National-Konsortium |0 StatID:(DE-HGF)0430 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b P NATL ACAD SCI USA : 2014 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b P NATL ACAD SCI USA : 2014 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-FRM-II-20110218 |k JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II |l JCNS-FRM-II |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-1-20110106 |k Neutronenstreuung ; JCNS-1 |l Neutronenstreuung |x 1 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-FRM-II-20110218 |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-1-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|