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@ARTICLE{MarchiArtzner:27634,
      author       = {Marchi-Artzner, V. and Lorz, B. and Gosse, C. and Jullien,
                      L. and Merkel, R. and Kessler, H. and Sackmann, M. J.},
      title        = {{A}dhesion of {A}rg-{G}ly-{A}sp ({RGD}) peptide vesicles
                      onto an integrin surface : visualization of the segregation
                      of {RGD} ligands into the adhesion plaques by fluorescence},
      journal      = {Langmuir},
      volume       = {19},
      issn         = {0743-7463},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {PreJuSER-27634},
      pages        = {835 - 841},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Integrins are adhesion receptors that mediate cell adhesion
                      and play an important function in many biological processes
                      such as morphogenesis and tissue remodeling. These membrane
                      proteins specifically interact with a short tripeptide
                      sequence, RGD (Arg-Gly-Asp), present in numerous
                      extracellular macromolecules. Model systems have been
                      developed in order to understand how membrane adhesion is
                      induced by this specific RGD peptide ligand/integrin
                      recognition system. We have previously shown that RGD giant
                      vesicles selectively adhere to endothelial cells by
                      formation of pinning centers. Nevertheless, the nature of
                      the lipids located in the adhesion contact zone is unknown.
                      One hypothesis is that the lipidic ligands migrate to the
                      contact zone where they are confined after binding to the
                      receptor. To study the possible formation of ligand domains
                      within the vesicle bilayer, we synthesized a fluorescently
                      labeled RGD lipid that can be easily incorporated in giant
                      vesicles. Adhesion of giant RGD vesicles onto an
                      integrin-functionalized surface was followed simultaneously
                      by reflection interference contrast microscopy and
                      fluorescence microscopy. For the first time, it was possible
                      to observe the microsegregation of RGD lipids in the contact
                      zone during adhesion. Additionally, we observed interesting
                      photosensitive properties of the chalcone chromophore that
                      could lead to a new method of analyzing the lipid
                      organization within the membrane during adhesion and to the
                      design of new ligand lipids and vesicle vectors for cell
                      targeting.},
      keywords     = {J (WoSType)},
      cin          = {ISG-4},
      ddc          = {670},
      cid          = {I:(DE-Juel1)VDB44},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK242},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Materials Science, Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000180737100055},
      doi          = {10.1021/la026227k},
      url          = {https://juser.fz-juelich.de/record/27634},
}