TY  - JOUR
AU  - Hersch, N.
AU  - Wolters, B.
AU  - Ungvari, Z.
AU  - Gautam, T.
AU  - Deshpande, D.
AU  - Merkel, R.
AU  - Csiszar, A.
AU  - Hoffmann, B.
AU  - Csiszar, A.
TI  - Biotin-conjugated fusogenic liposomes for high-quality cell purification
JO  - Journal of biomaterials applications
VL  - 30
IS  - 6
SN  - 1530-8022
CY  - Thousand Oaks, Calif. [u.a.]
PB  - Sage
M1  - FZJ-2015-06824
SP  - 846-856
PY  - 2016
AB  - Purification of defined cell populations from mixed primary cell sources is essential for many biomedical and biotechnological applications but often very difficult to accomplish due to missing specific surface markers. In this study, we developed a new approach for efficient cell population separation based on the specific membrane fusion characteristics of distinct cell types upon treatment with fusogenic liposomes. When such liposomes are conjugated with biotin, specific cell populations can be efficiently surface functionalized by biotin after liposomal treatment while other populations remain unlabeled. Due to the high affinity of biotin for avidin-like proteins, biotin functionalized cells are ideal targets for conjugation of e.g. avidin tagged magnetic beads, fluorophores or antibodies with bioanalytical relevance. Here, based on the differential biotinylation of distinct cell populations high quality separation of cardiac fibroblasts from myocytes, and cerebromicrovascular endothelial cells from fibroblasts was successfully established. 
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000367743400018
DO  - DOI:10.1177/0885328215603026
UR  - https://juser.fz-juelich.de/record/276366
ER  -