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@ARTICLE{Hersch:276366,
      author       = {Hersch, N. and Wolters, B. and Ungvari, Z. and Gautam, T.
                      and Deshpande, D. and Merkel, R. and Csiszar, A. and
                      Hoffmann, B. and Csiszar, A.},
      title        = {{B}iotin-conjugated fusogenic liposomes for high-quality
                      cell purification},
      journal      = {Journal of biomaterials applications},
      volume       = {30},
      number       = {6},
      issn         = {1530-8022},
      address      = {Thousand Oaks, Calif. [u.a.]},
      publisher    = {Sage},
      reportid     = {FZJ-2015-06824},
      pages        = {846-856},
      year         = {2016},
      abstract     = {Purification of defined cell populations from mixed primary
                      cell sources is essential for many biomedical and
                      biotechnological applications but often very difficult to
                      accomplish due to missing specific surface markers. In this
                      study, we developed a new approach for efficient cell
                      population separation based on the specific membrane fusion
                      characteristics of distinct cell types upon treatment with
                      fusogenic liposomes. When such liposomes are conjugated with
                      biotin, specific cell populations can be efficiently surface
                      functionalized by biotin after liposomal treatment while
                      other populations remain unlabeled. Due to the high affinity
                      of biotin for avidin-like proteins, biotin functionalized
                      cells are ideal targets for conjugation of e.g. avidin
                      tagged magnetic beads, fluorophores or antibodies with
                      bioanalytical relevance. Here, based on the differential
                      biotinylation of distinct cell populations high quality
                      separation of cardiac fibroblasts from myocytes, and
                      cerebromicrovascular endothelial cells from fibroblasts was
                      successfully established.},
      cin          = {ICS-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000367743400018},
      doi          = {10.1177/0885328215603026},
      url          = {https://juser.fz-juelich.de/record/276366},
}