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@ARTICLE{Braun:276367,
      author       = {Braun, Tobias and Kleusch, Christian and Naumovska, Elena
                      and Merkel, Rudolf and Csiszár, Agnes},
      title        = {{A} bioanalytical assay to distinguish cellular uptake
                      routes for liposomes},
      journal      = {Cytometry / A},
      volume       = {89},
      number       = {3},
      issn         = {1552-4922},
      address      = {Hoboken, NJ},
      publisher    = {Wiley-Liss},
      reportid     = {FZJ-2015-06825},
      pages        = {301-308},
      year         = {2016},
      abstract     = {Lipid-based nanoparticles are frequently used for drug or
                      DNA delivery into mammalian cells. However it is difficult
                      to determine whether such particles are taken up via
                      endocytosis or fusion to the plasma membrane. Here, we
                      propose a simple and reliable analytical method to do so
                      based on the unique spectral properties of the fluorescent
                      tracer BODIPY FL. At high local concentrations, this dye
                      displays an additional red-shifted emission peak that is
                      absent at low concentrations. In dye-loaded liposomes taken
                      up by endocytosis, the local dye concentration did not
                      significantly change upon internalization. Accordingly,
                      unchanged fluorescence spectra were detected. When cells
                      were incubated with liposomes able to fuse with the plasma
                      membrane of mammalian cells, a reduction of local dye
                      concentration and much weaker emission in the red-shifted
                      peak were observed. The ratio of intensities in both
                      fluorescence channels was shown to be a reliable indicator
                      of the cellular uptake mechanism. © 2015 International
                      Society for Advancement of Cytometry},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000374729900010},
      doi          = {10.1002/cyto.a.22792},
      url          = {https://juser.fz-juelich.de/record/276367},
}