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@ARTICLE{Goossens:276549,
      author       = {Goossens, Karel and Prior, Mira and Pacheco, Victor and
                      Willbold, Dieter and Müllen, Klaus and Enderlein, Jörg and
                      Hofkens, Johan and Gregor, Ingo},
      title        = {{A}ccurate {D}iffusion {C}oefficients of {O}rganosoluble
                      {R}eference {D}yes in {O}rganic {M}edia {M}easured by
                      {D}ual-{F}ocus {F}luorescence {C}orrelation {S}pectroscopy},
      journal      = {ACS nano},
      volume       = {9},
      number       = {7},
      issn         = {1936-086X},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2015-06925},
      pages        = {7360 - 7373},
      year         = {2015},
      abstract     = {Dual-focus fluorescence correlation spectroscopy (2fFCS) is
                      a versatile method to determine accurate diffusion
                      coefficients of fluorescent species in an absolute,
                      reference-free manner. Whereas (either classical or
                      dual-focus) FCS has been employed primarily in the life
                      sciences and thus in aqueous environments, it is
                      increasingly being used in materials chemistry, as well.
                      These measurements are often performed in nonaqueous media
                      such as organic solvents. However, the diffusion
                      coefficients of reference dyes in organic solvents are not
                      readily available. For this reason we determined the
                      translational diffusion coefficients of several commercially
                      available organosoluble fluorescent dyes by means of 2fFCS.
                      The selected dyes and organic solvents span the visible
                      spectrum and a broad range of refractive indices,
                      respectively. The diffusion coefficients can be used as
                      absolute reference values for the calibration of
                      experimental FCS setups, allowing quantitative measurements
                      to be performed. We show that reliable information about the
                      hydrodynamic dimensions of the fluorescent species
                      (including noncommercial compounds) within organic media can
                      be extracted from the 2fFCS data.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000358823200073},
      pubmed       = {pmid:26144863},
      doi          = {10.1021/acsnano.5b02371},
      url          = {https://juser.fz-juelich.de/record/276549},
}