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@ARTICLE{Kahlert:276571,
      author       = {Kahlert, Ulf Dietrich and Koch, Katharina and Suwala,
                      Abigail Kora and Hartmann, Rudolf and Cheng, Menglin and
                      Maciaczyk, Donata and Willbold, Dieter and Eberhart, Charles
                      G. and Glunde, Kristine and Maciaczyk, Jarek},
      title        = {{T}he effect of neurosphere culture conditions on the
                      cellular metabolism of glioma cells},
      journal      = {Folia neuropathologica},
      volume       = {3},
      issn         = {1641-4640},
      address      = {Pozna´n [u.a.]},
      publisher    = {Termedia Publ. House73588},
      reportid     = {FZJ-2015-06936},
      pages        = {219 - 225},
      year         = {2015},
      abstract     = {Glioblastoma (GBM) is the most common and lethal adult
                      glial brain tumour, with a mean overall survival of 16-19
                      months after primary diagnosis under the current
                      standard-of-care treatment scheme [26]. Despite enormous
                      research efforts towards early diagnosis and more efficient
                      treatment, the prognosis of GBMs remains dismal.The
                      influence of culture conditions has been wi­dely
                      investigated in the field of glioma research, suggesting
                      that neurosphere cultures, compared to adherent growth, more
                      closely resemble the original patient’s tumour [29]
                      showing high stem cell compartment [1] and therefore are
                      more suitable for testing of novel therapeutic spectras
                      approaches [30]. In this report we describe altered relative
                      concentrations of the cholines, creatine, myo-inositol, and
                      glycine in the human GBM cell line U87 propagated under stem
                      cell conditions as compared to classical monolayer culture.
                      Furthermore, U87 neurospheres showed significant higher
                      levels of the putative GBM stem cell marker CD133 as their
                      serum-propagated counterparts. Detection and targeting of
                      miss-regulated choline-, myo-inosi­tol-, creatine-, and
                      glycine-metabolism has been de­s­cribed to have potential
                      utility in the diagnosis and treatment of malignant gliomas
                      [2-4,13,16,19].This is, to our knowledge, the hitherto first
                      link of changes in those oncometabolites [4,25] to
                      variations in cell culture conditions of glioma cells. Inter
                      spectral co-analysis of metabolite concentrations under the
                      two propagation conditions identified reductions in ratios
                      of phosphocholine to glycerophosphocholine (PC/GPC) and
                      glycine to total choline (Gly/tCho) but increases in the
                      quotient of total choline to total creatine (tCho/tCre) and
                      PC/tCre, as well as Gly/myo-inositol (Gly/myo). This work
                      should draw the attention of the scientific community on
                      possible in vitro artefacts and on the need for appropriate
                      models most closely resembling the in vivo biology of
                      investigated tumours.},
      cin          = {ICS-6},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000362463100004},
      doi          = {10.5114/fn.2015.54422},
      url          = {https://juser.fz-juelich.de/record/276571},
}