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@ARTICLE{Brger:276620,
author = {Börger, Claudia and Schünke, Sven and Lecher, Justin and
Stoldt, Matthias and Winkhaus, Friederike and Kaupp, U.
Benjamin and Willbold, Dieter},
title = {{R}esonance assignment of the ligand-free cyclic
nucleotide-binding domain from the murine ion channel
{HCN}$_{2}$},
journal = {Biomolecular NMR assignments},
volume = {9},
number = {2},
issn = {1874-270X},
address = {Dordrecht [u.a.]},
publisher = {Springer Netherlands},
reportid = {FZJ-2015-06965},
pages = {243 - 246},
year = {2015},
abstract = {Hyperpolarization activated and cyclic nucleotide-gated
(HCN) ion channels as well as cyclic nucleotide-gated (CNG)
ion channels are essential for the regulation of cardiac
cells, neuronal excitability, and signaling in sensory
cells. Both classes are composed of four subunits. Each
subunit comprises a transmembrane region, intracellular N-
and C-termini, and a C-terminal cyclic nucleotide-binding
domain (CNBD). Binding of cyclic nucleotides to the CNBD
promotes opening of both CNG and HCN channels. In case of
CNG channels, binding of cyclic nucleotides to the CNBD is
sufficient to open the channel. In contrast, HCN channels
open upon membrane hyperpolarization and their activity is
modulated by binding of cyclic nucleotides shifting the
activation potential to more positive values. Although
several high-resolution structures of CNBDs from HCN and CNG
channels are available, the gating mechanism for murine HCN2
channel, which leads to the opening of the channel pore, is
still poorly understood. As part of a structural
investigation, here, we report the complete backbone and
side chain resonance assignments of the murine HCN2 CNBD
with part of the C-linker.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000361440100005},
doi = {10.1007/s12104-014-9583-x},
url = {https://juser.fz-juelich.de/record/276620},
}