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@ARTICLE{Hung:276643,
      author       = {Hung, Yu-Fu and Schwarten, Melanie and Hoffmann, Silke and
                      Willbold, Dieter and Sklan, Ella and König, Bernd},
      title        = {{A}mino {T}erminal {R}egion of {D}engue {V}irus {NS}4{A}
                      {C}ytosolic {D}omain {B}inds to {H}ighly {C}urved
                      {L}iposomes},
      journal      = {Viruses},
      volume       = {7},
      number       = {7},
      issn         = {1999-4915},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2015-06971},
      pages        = {4119 - 4130},
      year         = {2015},
      abstract     = {Dengue virus (DENV) is an important human pathogen causing
                      millions of disease cases and thousands of deaths worldwide.
                      Non-structural protein 4A (NS4A) is a vital component of the
                      viral replication complex (RC) and plays a major role in the
                      formation of host cell membrane-derived structures that
                      provide a scaffold for replication. The N-terminal
                      cytoplasmic region of NS4A(1–48) is known to
                      preferentially interact with highly curved membranes. Here,
                      we provide experimental evidence for the stable binding of
                      NS4A(1–48) to small liposomes using a liposome floatation
                      assay and identify the lipid binding sequence by NMR
                      spectroscopy. Mutations L6E;M10E were previously shown to
                      inhibit DENV replication and to interfere with the binding
                      of NS4A(1–48) to small liposomes. Our results provide new
                      details on the interaction of the N-terminal region of NS4A
                      with membranes and will prompt studies of the functional
                      relevance of the curvature sensitive membrane anchor at the
                      N-terminus of NS4A.},
      cin          = {ICS-6},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000360353200038},
      pubmed       = {pmid:26197333},
      doi          = {10.3390/v7072812},
      url          = {https://juser.fz-juelich.de/record/276643},
}