%0 Journal Article
%A Schwarten, Melanie
%A Mohrlüder, Jeannine
%A Ma, Peixiang
%A Stoldt, Matthias
%A Thielmann, Yvonne
%A Stangler, Thomas
%A Hersch, Nils
%A Hoffmann, Bernd
%A Merkel, Rudolf
%A Willbold, Dieter
%T Nix directly binds to GABARAP: A possible crosstalk between apoptosis and autophagy
%J Autophagy
%V 5
%N 5
%@ 1554-8635
%C Abingdon, Oxon
%I Taylor & Francis
%M FZJ-2015-06975
%P 690 - 698
%D 2009
%X Autophagy, a pathway primarily relevant for cell survival, and apoptosis, a process invariably leading to cell death, are the two main mechanisms of cellular self-destruction, which are essential in cell growth, neurodegeneration, tumor suppression, stress and immune response. Currently, a potential crosstalk between apoptosis and autophagy is subject to intensive investigations since recently some direct junctions became obvious. The respective protein-protein interaction network, however, remains to be elucidated in detail. The γ-aminobutyric acid type A (GABAA) receptor-associated protein GABARAP belongs to a family of proteins implicated in intracellular transport events and was shown to be associated to autophagic processes. Using a phage display screening against the target protein GABARAP, we identified the proapoptotic protein Nix/Bnip3L to be a potential GABARAP ligand. In vitro binding studies, pull-down analysis,coimmunoprecipitation assays and colocalization studies confirmed a direct interaction of both proteins in mammalian cells.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:19363302
%U <Go to ISI:>//WOS:000268205300011
%R 10.4161/auto.5.5.8494
%U https://juser.fz-juelich.de/record/276647