TY  - JOUR
AU  - Schwarten, Melanie
AU  - Mohrlüder, Jeannine
AU  - Ma, Peixiang
AU  - Stoldt, Matthias
AU  - Thielmann, Yvonne
AU  - Stangler, Thomas
AU  - Hersch, Nils
AU  - Hoffmann, Bernd
AU  - Merkel, Rudolf
AU  - Willbold, Dieter
TI  - Nix directly binds to GABARAP: A possible crosstalk between apoptosis and autophagy
JO  - Autophagy
VL  - 5
IS  - 5
SN  - 1554-8635
CY  - Abingdon, Oxon
PB  - Taylor & Francis
M1  - FZJ-2015-06975
SP  - 690 - 698
PY  - 2009
AB  - Autophagy, a pathway primarily relevant for cell survival, and apoptosis, a process invariably leading to cell death, are the two main mechanisms of cellular self-destruction, which are essential in cell growth, neurodegeneration, tumor suppression, stress and immune response. Currently, a potential crosstalk between apoptosis and autophagy is subject to intensive investigations since recently some direct junctions became obvious. The respective protein-protein interaction network, however, remains to be elucidated in detail. The γ-aminobutyric acid type A (GABAA) receptor-associated protein GABARAP belongs to a family of proteins implicated in intracellular transport events and was shown to be associated to autophagic processes. Using a phage display screening against the target protein GABARAP, we identified the proapoptotic protein Nix/Bnip3L to be a potential GABARAP ligand. In vitro binding studies, pull-down analysis,coimmunoprecipitation assays and colocalization studies confirmed a direct interaction of both proteins in mammalian cells.
LB  - PUB:(DE-HGF)16
C6  - pmid:19363302
UR  - <Go to ISI:>//WOS:000268205300011
DO  - DOI:10.4161/auto.5.5.8494
UR  - https://juser.fz-juelich.de/record/276647
ER  -